Theoretical Modeling of Aging Effects in Microtubule Dynamics
Theoretical Modeling of Aging Effects in Microtubule Dynamics
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DOI：
10.1016/j.bpj.2010.11.047
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发表时间：
2011-02-16
影响因子：
3.4
通讯作者：
Santen, Ludger
中科院分区：
文献类型：
--
作者：
Ebbinghaus, Maximilian;Santen, Ludger
微管(MT)网络是细胞骨架的重要组成部分，随着单丝生长和收缩的交替变化，微管网络不断重塑。加端跟踪蛋白(+TIPS)与MT相互作用，并在许多情况下改变其动力学。尽管已经确定一些+TIPS通过增加救援来修改MT的动态，但加端跟踪机制仍在争论中。我们提出了一种MT动力学模型，在该模型中，在生长过程中向丝状结构动态添加了一个救援因子。因此，灯丝显示的老化行为应该是实验上可以接触到的，因此可以排除一些关于MT Plus端包含救援因素的假设模型。这一结果不仅限于+TIPS，而且可以推广到任何一种改变动力不稳定性参数的机构。此外，我们还发现晶胞几何形状对定量结果有很大的影响。
The microtubule (MT) network, an important part of the cytoskeleton, is constantly remodeled by alternating phases of growth and shrinkage of individual filaments. Plus-end tracking proteins (+TIPs) interact with the MT and in many cases alter its dynamics. Although it is established that some +TIPs modify MT dynamics by increasing rescues, the plus-end tracking mechanism is still under debate. We present a model for MT dynamics in which a rescue factor is dynamically added to the filament during growth. As a consequence, the filament shows aging behavior that should be experimentally accessible and thus allow one to exclude some hypothesized models regarding the inclusion of rescue factors at the MT plus end. This result is not limited to +TIPs and can be extended to any kind of mechanism shifting the parameters of dynamic instability. Additionally, we show that the cell geometry has a strong influence on the quantitative results.