QTL mapping of fruit-related traits in pepper (Capsicum annuum)
QTL mapping of fruit-related traits in pepper (Capsicum annuum)
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DOI：
10.1007/s001220000461
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发表时间：
2001-05-01
影响因子：
5.4
通讯作者：
Peleman, J
中科院分区：
文献类型：
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作者：
Ben Chaim, A;Paran, I;Peleman, J
对铃型辣椒品种毛尔和印度小果系多年生辣椒杂交F_1群体的果实性状进行了QTL分析。利用RFLP、AFLP(R)1、RAPD和形态标记(共177个)构建了辣椒-番茄杂交组合的比较遗传图谱，并对180个FG家系的14个数量遗传性状进行了评价。以LOD 3.0作为QTL检测的阈值，进行区间分析，共检测到55个QTL。果实直径和重量、果皮厚度和花梗直径等性状的QTL往往位于相似的染色体区域，反映了这些性状之间的高度遗传相关。在3号染色体上检测到一个主效QTL，该QTL可解释果实形状(果长与果径比)表型变异的60%以上。该染色体还含有群体中多数性状的QTL。连锁群2、3、8和10中的标记与多个性状的QTL相关，表明它们作为控制辣椒发育过程的基因座具有重要意义。辣椒中的几个QTL似乎与控制相同性状的基因座在番茄中的位置相对应，这表明这些QTL可能在两个物种中是同源的。
QTL analysis of pepper fruit characters was performed in an F, population derived from a cross between two Capsicum annum genotypes, the bell-type cultivar Maor and the Indian small-fruited line Perennial. RFLP, AFLP((R)1), RAPD and morphological markers (a total of 177) were used to construct a comparative pepper-tomato genetic map for this cross, and 14 quantitatively inherited traits were evaluated in 180 Fg families. A total of 55 QTL were identified by interval analysis using LOD 3.0 as the threshold for QTL detection. QTL for several traits including fruit diameter and weight, pericarp thickness and pedicel diameter were often located in similar chromosomal regions, thus reflecting high genetic correlations among these traits. A major QTL that accounts for more than 60% of the phenotypic variation for fruit shape (ratio of fruit length to fruit diameter) was detected in chromosome 3. This chromosome also contained QTL for most of the traits scored in the population. Markers in linkage groups 2, 3, 8 and 10 were associated with QTL for multiple traits, thereby suggesting their importance as loci that control developmental processes in pepper. Several QTL in pepper appeared to correspond to positions in tomato for loci controlling the same traits, suggesting the hypothesis that these QTL may be orthologous in the two species.