Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients
Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients
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COVID-19患者支气管肺泡灌洗液和外周血单个核细胞的转录组特征
DOI：
10.1080/22221751.2020.1747363
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发表时间：
2020-01-01
影响因子：
13.2
通讯作者：
Chen, Yu
中科院分区：
文献类型：
--
作者：
Xiong, Yong;Liu, Yuan;Chen, Yu
摘要正在中国和其他158个国家和地区流行的新冠肺炎疫情已造成毁灭性的死亡，并对公众健康构成极大威胁。然而，由于我们对这种致命疾病的宿主免疫反应的了解有限，寻找有效的支持性治疗药物和治疗方法的努力一直受到阻碍。为了研究宿主对SARS-CoV-2感染的炎症反应的转录特征，我们对从新冠肺炎患者的支气管肺泡灌洗液和外周血单个核细胞中提取的RNA进行了转录组测序。我们的结果揭示了患者感染SARS-CoV-2的不同宿主炎症细胞因子的特征，并强调了新冠肺炎发病与过度释放细胞因子如CCL2/MCP-1、CXCL10/IP-10、CCL3/MIP-1A和CCL4/MIP1B之间的关系。此外，SARS-CoV-2诱导的淋巴细胞凋亡和P53信号通路的激活可能是患者淋巴细胞减少的原因。新冠肺炎患者的转录组数据集将为抗炎药物的临床指导和了解宿主反应的分子机制提供有价值的资源。
ABSTRACT Circulating in China and 158 other countries and areas, the ongoing COVID-19 outbreak has caused devastating mortality and posed a great threat to public health. However, efforts to identify effectively supportive therapeutic drugs and treatments has been hampered by our limited understanding of host immune response for this fatal disease. To characterize the transcriptional signatures of host inflammatory response to SARS-CoV-2 (HCoV-19) infection, we carried out transcriptome sequencing of the RNAs isolated from the bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) specimens of COVID-19 patients. Our results reveal distinct host inflammatory cytokine profiles to SARS-CoV-2 infection in patients, and highlight the association between COVID-19 pathogenesis and excessive cytokine release such as CCL2/MCP-1, CXCL10/IP-10, CCL3/MIP-1A, and CCL4/MIP1B. Furthermore, SARS-CoV-2 induced activation of apoptosis and P53 signalling pathway in lymphocytes may be the cause of patients’ lymphopenia. The transcriptome dataset of COVID-19 patients would be a valuable resource for clinical guidance on anti-inflammatory medication and understanding the molecular mechansims of host response.