Tumor Necrosis Factor-Alpha and 8-Hydroxy-2'-Deoxyguanosine are
Tumor Necrosis Factor-Alpha and 8-Hydroxy-2'-Deoxyguanosine are
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DOI：
10.1159/000368510
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Zha
中科院分区：
文献类型：
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作者：
He FF;Li HQ;Huang QX;Wang QY;Jiang HJ;Chen S;Su H;Zha
背景/目的我们以前发现糖尿病患者尿和血清血管生成素-2（Angiopoietin-2，Ang-2）水平随蛋白尿程度的加重而显著升高，但其原因尚不清楚。因此，我们的目的是确定是否有一个协会之间的Ang-2，炎症细胞因子（TNF-α和IL-18）和活性氧（8-OHdG和SOD）在2型糖尿病患者的蛋白尿。MethodsThis回顾性研究评估了113例2型糖尿病患者和normoalbuminuria，微量白蛋白尿，或macroalbuminuria和30名健康对照。ELISA法检测血清和尿液TNF-α、IL-18和8-OHdG水平。结果血清和尿液TNF-α、IL-18和8-OHdG水平随蛋白尿程度的加重而显著升高，并与Ang-2的升高呈正相关。相反，SOD活性随着蛋白尿的程度而降低，并与Ang-2呈负相关。多元线性回归分析显示，血清Ang-2水平与TNF-α（P< 0.001）、8-OHdG（P= 0.001）和IL-18（P= 0.003）水平独立相关。结论TNF-α、8-OHdG与2型糖尿病伴白蛋白尿患者尿Angiopoietin-2水平升高有关。
Background/AimsWe previously showed that urine and serum Angiopoietin-2 (Ang-2) levels increased significantly with the degree of albuminuria in diabetes patients, but the reasons remain unclear. Consequently we aimed to determine whether there was an association between Ang-2, inflammatory cytokines (TNF-α and IL-18) and reactive oxygen species (8-OHdG and SOD) in type 2 diabetes patients with albuminuria.MethodsThis retrospective study evaluated 113 patients with type 2 diabetes and normoalbuminuria, microalbuminuria, or macroalbuminuria and 30 healthy controls. Serum and urine TNF-α, IL-18 and 8-OHdG levels were measured by ELISA. Superoxide dismutase (SOD) activity was determined by spectrophotometry.ResultsSerum and urine TNF-α, IL-18 and 8-OHdG levels increased significantly with the degree of albuminuria, and were positively correlated with increased Ang-2. In contrast, SOD activity decreased with the degree of albuminuria and was negatively correlated with Ang-2. Multivariable linear regression analysis revealed that serum Ang-2 level was independently associated with serum levels of TNF-α (P< 0.001), 8-OHdG (P= 0.001), and IL-18 (P= 0.003). Urinary Ang-2 level was independently associated with urinary TNF-α (P< 0.001) and 8-OHdG (P= 0.004) levels.ConclusionTNF-α and 8-OHdG are associated with elevated urinary Angiopoietin-2 levels in type 2 diabetic patients with albuminuria.