TYPE-1 FIMBRIAE MUTANTS OF ESCHERICHIA-COLI K12 - CHARACTERIZATION OF RECOGNIZED AFIMBRIATE STRAINS AND CONSTRUCTION OF NEW FIM DELETION MUTANTS
TYPE-1 FIMBRIAE MUTANTS OF ESCHERICHIA-COLI K12 - CHARACTERIZATION OF RECOGNIZED AFIMBRIATE STRAINS AND CONSTRUCTION OF NEW FIM DELETION MUTANTS
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DOI：
10.1111/j.1365-2958.1991.tb00790.x
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发表时间：
1991-06-01
影响因子：
3.6
通讯作者：
EISENSTEIN, BI
中科院分区：
文献类型：
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作者：
BLOMFIELD, IC;MCCLAIN, MS;EISENSTEIN, BI
我们已经使用Southern杂交分析来表征大肠杆菌K12，包括菌株HB 101，P678-54和VL 584的1型菌毛突变体中的fim同源性的程度。 我们已经在菌株HB 101中发现了广泛的同源性，并证实P678-54缺乏大部分fim DNA。 菌株VL 584含有整个fim区的缺失。 我们已经使用了一种新的等位基因交换程序来产生菌株MG 1655、MM 294和YMC 9的新型fim缺失衍生物。 为了增加新缺失菌株的效用，我们还分离了每个突变体的recA衍生物。 这些菌株有利于分离、鉴定和操作来自不同来源的克隆菌毛基因。
We have used Southern hybridization analysis to characterize the extent of fim homology in recognized type 1 fimbriae mutants of Escherichia coli K12, including strains HB101, P678-54, and VL584. We have found extensive homology in strain HB101, and confirm that P678-54 lacks the majority of fim DNA. Strain VL584 contains a deletion of the entire fim region. We have used a new allelic exchange procedure to generate novel fim deletion derivatives of strains MG1655, MM294, and YMC9. To increase the utility of the new deletion strains we also isolated recA derivatives of each mutant. These strains facilitate the isolation, characterization, and manipulation of cloned fimbriae genes from diverse sources.