Monocarboxylate transporter expression in mouse brain
Monocarboxylate transporter expression in mouse brain
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DOI：
10.1152/ajpendo.1998.275.3.e516
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发表时间：
1998-09-01
影响因子：
5.1
通讯作者：
Landschulz, WH
中科院分区：
文献类型：
--
作者：
Koehler-Stec, EM;Simpson, IA;Landschulz, WH
虽然葡萄糖是正常大脑功能所需的主要代谢燃料，但单羧酸，即乳酸、丙酮酸和酮体，也可以被大脑用作替代能量底物。在大多数哺乳动物细胞中，这些底物通过单羧酸转运蛋白 (MCT) 家族转运入或转运出细胞，该家族首先在仓鼠中进行克隆和测序。我们最近从小鼠肾脏 cDNA 文库中克隆了两种 MCT 亚型（MCT1 和 MCT2）。 Northern 印迹分析显示 MCT1 mRNA 普遍存在，并且可以在大多数组织中以相对恒定的水平检测到。 MCT2 表达较为有限，高水平表达仅限于睾丸、肾脏、胃和肝脏，而在肺、脑和附睾脂肪中表达水平较低。使用反义核糖核酸探针和原位杂交在小鼠大脑中检测 MCT1 mRNA 和 MCT2 mRNA。 MCT1 mRNA 遍布整个皮质，在海马和小脑中杂交水平较高。在相同区域检测到 MCT2 mRNA，但表达模式更加特异。此外，MCT1 mRNA（而非 MCT2）定位于脉络丛、室管膜、微血管和白质结构（如胼胝体）。这些结果表明两种 MCT 在细胞水平上存在差异表达。
Although glucose is the major metabolic fuel needed for normal brain function, monocarboxylic acids, i.e., lactate, pyruvate, and ketone bodies, can also be utilized by the brain as alternative energy substrates. In most mammalian cells, these substrates are transported either into or out of the cell by a family of monocarboxylate transporters (MCTs), first cloned and sequenced in the hamster. We have recently cloned two MCT isoforms (MCT1 and MCT2) from a mouse kidney cDNA library. Northern blot analysis revealed that MCT1 mRNA is ubiquitous and can be detected in most tissues at a relatively constant level. MCT2 expression is more limited, with high levels of expression confined to testes, kidney, stomach, and liver and lower levels in lung, brain, and epididymal fat. Both MCT1 mRNA and MCT2 mRNA are detected in mouse brain using antisense riboprobes and in situ hybridization. MCT1 mRNA is found throughout the cortex, with higher levels of hybridization in hippocampus and cerebellum. MCT2 mRNA was detected in the same areas, but the pattern of expression was more specific. In addition, MCT1 mRNA, but not MCT2, is localized to the choroid plexus, ependyma, microvessels, and white matter structures such as the corpus callosum. These results suggest a differential expression of the two MCTs at the cellular level.