mRNA expression of cytokines and chemokines in herniated lumbar intervertebral discs
mRNA expression of cytokines and chemokines in herniated lumbar intervertebral discs
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DOI：
10.1097/00007632-200205010-00005
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发表时间：
2002-05-01
期刊：
影响因子：
3
通讯作者：
Kim, HS
中科院分区：
文献类型：
--
作者：
Ahn, SH;Cho, YW;Kim, HS
研究设计。目的研究细胞因子和趋化因子在腰椎间盘突出症标本中的表达。目的：研究IL-1α、肿瘤坏死因子-α、RANTES、IL-8、IL-10和转化生长因子-β的mRNAs在手术获得的腰椎间盘突出症标本中是否表达，并发现其中哪些是与临床症状和体征相关的主要细胞因子，以及不同类型的腰椎间盘突出症是否存在mRNA表达的差异。据推测，细胞因子参与了腰椎间盘突出症引起神经根性腿痛的过程。尽管关于IL-1α和TNF-α对腰椎间盘突出症的研究很少，但对于细胞因子和趋化因子的研究几乎还没有。采用逆转录-聚合酶链式反应技术，对突出的腰椎间盘标本中细胞因子和趋化因子的表达进行了研究。描述直腿抬高试验、背伸神经根性疼痛的发展、症状持续时间、视觉模拟评分的疼痛强度和突出的类型。IL-8、TNF-α、IL-1α、RANTES和IL-10的mRNAs分别在16例(70%)、15例(65%)、9例(39%)、4例(17%)和2例(9%)中表达。10例标本中有5例(50%)表达转化生长因子-β基因。IL-8mRNA的表达与神经根伸背痛的发生和症状持续时间短(平均3.8周)有关。IL-1α的mRNAs在间质延伸区的表达高于在基底层延伸区的表达，但表达较弱。白介素8似乎与背部伸展引起的神经根性疼痛有关，并在急性或亚急性腰椎间盘突出症时被激活。IL-8似乎参与了腰椎间盘突出症神经根炎的发病机制，提示其可能被认为是治疗干预的靶点。
Study Design. The mRNA expressions of cytokines and chemokines were assessed in herniated lumbar disc specimens.Objectives. To investigate whether the mRNAs of interleukin (IL)-1alpha, tumor necrosis factor (TNF)-alpha, RANTES, IL-8, IL-10, and transforming growth factor (TGF)-beta are expressed in surgically obtained herniated disc specimens; and to discover which of them are the predominant cytokines associated with the clinical symptoms and signs, and whether any differences in the mRNA expression exist depending on the different types of disc herniations.Summary of Background Data. It has been postulated that cytokines are involved in causing radicular leg pain in lumbar disc herniations. Although a few studies have been done on lumbar disc herniations concerning IL-1alpha and TNF-alpha, almost none has been carried out in the cases of the other of cytokines and chemokines.Methods. Using a reverse transcription-polymerase chain reaction, mRNA expressions of cytokines and chemokines were investigated in herniated disc specimens. The straight leg raising test, development of radicular pain by back extension, symptom duration, pain intensity using a visual analogue scale, and herniation types were described.Results. The mRNAs of IL-8, TNF-alpha, IL-1alpha, RANTES, and IL-10 were expressed in 16 (70%), 15 (65%), 9 (39%), 4 (17%), and 2 (9%) of the 23 herniated disc specimens, respectively. The mRNA of TGF-beta was expressed in 5 of 10 specimens (50%). IL-8 mRNA expression was associated with the development of radicular pain by back extension and short symptom duration (average 3.8 weeks). The mRNAs of IL-1alpha were expressed more frequently in transligamentous extensions than in subligamentous extensions, but the expression was weak.Conclusion. Interleukin-8 appears to be associated with development of radicular pain by back extension and to be activated on acute or subacute disc herniations. IL-8 seems to participate in the pathomechanism of nerve root inflammation in lumbar disc herniations, which implies that it may be considered a target for therapeutic intervention.