Dynamics and function of ERM proteins during cytokinesis in human cells
Dynamics and function of ERM proteins during cytokinesis in human cells
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DOI：
10.1002/1873-3468.12844
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发表时间：
2017-10-01
期刊：
影响因子：
3.5
通讯作者：
Uehara, Ryota
中科院分区：
文献类型：
--
作者：
Hiruma, Shota;Kamasaki, Tomoko;Uehara, Ryota
在动物细胞质分裂过程中控制细胞骨架-膜相互作用的分子机制仍然不清楚。在这里，我们研究了ERM(Ezrin/Radioxin/Moesin)蛋白在人类培养细胞胞质分裂过程中的动态和功能。我们发现Ezrin通过其膜相关结构域以一种胆固醇依赖但在很大程度上不依赖Rho的方式被招募到卵裂沟中。虽然ERM对于沟槽内移来说是可有可无的，但它们在极地皮质的收缩活动中起着关键作用。值得注意的是，当香草素和超绒毛蛋白被耗尽时，内质网在卵裂沟处积累越来越多，并在很大程度上促进了沟道的深入。这些结果揭示了ERM在胞质分裂过程中对皮质活动的支持作用，并为优先将胞质分裂相关蛋白与分裂部位联系在一起的选择机制提供了洞察力。
The molecular mechanism that governs cytoskeleton-membrane interaction during animal cytokinesis remains elusive. Here, we investigated the dynamics and functions of ERM (Ezrin/Radixin/Moesin) proteins during cytokinesis in human cultured cells. We found that ezrin is recruited to the cleavage furrow through its membrane-associated domain in a cholesterol-dependent but largely Rho-independent manner. While ERMs are dispensable for furrow ingression, they play a pivotal role in contractile activity of the polar cortex. Notably, when anillin and supervillin are codepleted, ERMs increasingly accumulate at the cleavage furrow and substantially contribute to the furrow ingression. These results reveal a supportive role of ERMs in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis-relevant proteins with the division site.