Association of HTLV-I antibody profile of asymptomatic carriers with proviral DNA levels of peripheral blood mononuclear cells.
Association of HTLV-I antibody profile of asymptomatic carriers with proviral DNA levels of peripheral blood mononuclear cells.
复制标题
无症状携带者的HTLV-I抗体谱与外周血单核细胞的前病毒DNA水平的关联。
DOI：
--
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
H. Tsubouchi
中科院分区：
文献类型：
--
作者：
S. Ishihara;A. Okayama;S. Stuver;H. Horinouchi;S. Shioiri;K. Murai;T. Kubota;R. Yamashita;N. Tachibana;H. Tsubouchi
对日本宫崎市216例无症状携带者的人类嗜T淋巴细胞病毒I型（HTLV-I）抗体谱进行了分析，并结合半定量聚合酶链反应（PCR）方法测定了其外周血单个核细胞（PBMC）中HTLV-I前病毒DNA水平。58例（27%）DNA水平较高的受试者（PA）的颗粒凝集试验（PA）的几何平均HTLV-I滴度为1：1，240; 94例（44%）DNA水平中等，为1：740; 38例（18%）DNA水平较低，为1：476; 26例（12%）DNA水平不可检测，为1：263。而且，当受试者按滴度从高到低分为四组时，DNA水平与抗体滴度水平之间的相关性非常显著（p < 0.0001）。随机抽取78例携带者血清，用Western blot法检测其抗HTLV-Ⅰ抗体亚类。免疫球蛋白（IG）M抗体阳性35例（45%）。在每个可检测的前病毒DNA水平上，IgM抗体携带者的平均PA抗体滴度高于无IgM抗体携带者。这些结果表明，HTLV-Ⅰ抗体滴度与前病毒DNA水平有关，也与前病毒DNA水平相同的人中IgM抗体的存在有关。WB检测IgG抗体阴性者7例（9%），其中前病毒DNA水平低或检测不到，PA滴度也低。其中3例仅IgM抗体阳性。
The human T-lymphotropic virus type I (HTLV-I) antibody profile of 216 asymptomatic carriers in Miyazaki, Japan, was analyzed in conjunction with the HTLV-I proviral DNA levels in their peripheral blood mononuclear cells (PBMC) determined by the semiquantitative polymerase chain reaction (PCR) method. The geometric mean HTLV-I titer by particle agglutination assay (PA) of 58 subjects (27%) with a high DNA level was 1:1, 240; 94 (44%) with a medium DNA level, 1:740; 38 (18%) with a low level, 1:476; and, 26 (12%) with an undetectable level, 1:263. Moreover, when the subjects were divided into four groups according to titer from high to low, the correlation between DNA level and antibody titer level was highly significant (p < 0.0001). HTLV-I antibody subclass by Western blot (WB) was determined for 78 randomly selected samples from these carriers. Immunoglobulin (Ig) M antibody was detected in 35 (45%). The mean PA antibody titer was higher in carriers with IgM antibody than in those without, at each detectable proviral DNA level. These findings suggest that HTLV-I antibody titer is related to proviral DNA level and also to the presence of IgM antibodies among those with proviral DNA of the same level. Seven carriers (9%) were negative for IgG antibody by WB, among whom the proviral DNA level was low or undetectable and the PA titer was also low. Three of these were positive only for IgM antibody.