Accumulation of catechins in tea in relation to accumulation of mRNA from genes involved in catechin biosynthesis
Accumulation of catechins in tea in relation to accumulation of mRNA from genes involved in catechin biosynthesis
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DOI：
10.1016/j.plaphy.2008.11.002
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发表时间：
2009-02-01
影响因子：
6.5
通讯作者：
Popluechai, S.
中科院分区：
文献类型：
--
作者：
Eungwanichayapant, P. D.;Popluechai, S.
儿茶素是茶树中发现的一组多酚类物质。中国)处于较高水平。它们有益于健康。用高效液相色谱法对乌龙17号茶树枝条和成熟叶片中儿茶素的积累进行了研究，结果表明，茶树枝条和成熟叶片中儿茶素的含量大多高于成熟叶片，只有没食子儿茶素没食子酸酯(CG)在枝条和成熟叶片中有微量存在。用逆转录聚合酶链式反应(RT-PCR)研究儿茶素合成相关基因的mRNA积累。结果表明，这些基因在新梢中的mRNA积累量高于成熟叶片。这些基因包括苯丙氨酸解氨酶1(PAL1；EC 4.3.1.5)、查尔酮合成酶(CHS；EC 2.3.1.74)、二氢黄酮醇4-还原酶(DFR；EC 1.1.1.219)、亮氨酸花青素还原酶(LCR；EC 1.17.1.3)和黄烷酮3-羟基酶(F3H；EC 1.14.11.9)基因。(C)2008年爱思唯尔·马森公司。版权所有。
Catechins are a group of polyphenols found in tea (Camellia sinensis var. sinensis) at high levels. They are beneficial for health. From the study on accumulation of catechins in shoots and mature leaves of a tea cultivar, Oolong No. 17, using high-performance liquid chromatography (HPLC), it was found that the amounts of most catechins in the shoots were higher than those in the mature leaves, with an exception of catechins gallate (CG) that was found in trace amounts in both the shoots and mature leaves. mRNA accumulation of genes involved in catechin synthesis was studied using reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that the mRNA accumulation of the genes were higher in the shoots than in the mature leaves. These genes included genes of phenylalanine ammonialyase 1 (PAL1; EC 4.3.1.5), chalcone synthase (CHS; EC 2.3.1.74), dihydroflavonol 4-reductase (DFR; EC 1.1.1.219), leucoanthocyanidin reductase (LCR; EC 1.17.1.3), and flavanone 3-hydroxylase (F3H; EC 1.14.11.9). (C) 2008 Elsevier Masson SAS. All rights reserved.