Immunohistochemical and western analyses of protein arginine N-methyltransferase 3 in the mouse brain
Immunohistochemical and western analyses of protein arginine N-methyltransferase 3 in the mouse brain
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DOI：
10.1016/j.neuroscience.2006.05.022
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Tohyama, M.
中科院分区：
文献类型：
--
作者：
Ikenaka, K.;Miyata, S.;Tohyama, M.
使用间接免疫荧光法研究了小鼠大脑中蛋白质精氨酸 N-甲基转移酶 3 (PRMT3) 的分布。观察到 PRMT3 位于神经元的细胞体和树突中，但不在轴突和神经胶质细胞中，表明 PRMT3 参与神经元功能。大脑中免疫反应神经元的分布不均匀，表明PRMT3在特定的神经元系统如运动和边缘系统以及与小脑相关的功能中发挥作用。目前使用蛋白质印迹方法对 PRMT1 和 PRMT3 进行的个体发育分析清楚地表明，PRMT3 在围产期发育，甚至在成年期也保持表达。另一方面，PRMT1 在早期胚胎阶段短暂表达。这些发现表明，PRMT3 与年轻和成人大脑中的神经元功能相关，而 PRMT1 在未成熟的大脑中发挥作用，例如神经回路的形成。 (c) 2006 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
The distribution of protein arginine N-methyltransferase 3 (PRMT3) was investigated in the mouse brain using indirect immunofluorescence. PRMT3 was observed to be localized in the cell bodies and dendrites of neurons but not in the axons and glial cells, indicating that PRMT3 is involved in neuronal function. The distribution of the immunoreactive neurons in the brain was uneven, indicating that PRMT3 plays a role in specific neuronal systems such as the motor and limbic systems, as well as functions related to the cerebellum. The present ontogenetic analysis of PRMT1 and PRMT3 using Western blot methodology clearly revealed that PRMT3 develops during the perinatal stage and its expression is maintained even in adulthood. PRMT1, on the other hand, is expressed transiently during the early embryonic stage. These findings indicate that PRMT3 is related with neuronal function in both young and adult brains, while PRMT1 has roles in the immature brain, such as the formation of neural circuits. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.