High-throughput SPR sensor for food safety
High-throughput SPR sensor for food safety
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DOI：
10.1016/j.bios.2008.08.012
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发表时间：
2009-01-01
影响因子：
12.6
通讯作者：
Homola, Jiri
中科院分区：
文献类型：
--
作者：
Piliarik, Marek;Parova, Lucie;Homola, Jiri
报道了一种高通量的表面等离子体共振（SPR）生物传感器，用于快速、并行地检测特定细菌病原体的核酸。该生物传感器由具有偏振对比度和内部参考（折射率分辨率2 × 10（-7）RIU）的高性能SPR成像传感器和在SPR传感器表面上微斑点的DNA探针阵列组成。已证明，在100 pM水平下可检测到细菌病原体（如流产布鲁氏菌、大肠杆菌和金黄色葡萄球菌）的特征性核酸短序列（20-23个碱基）。该SPR传感器可在15 min内完成对特定DNA或RNA序列的检测。(C)2008 Elsevier B. V.保留所有权利。
High-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of nucleic acids identifying specific bacterial pathogens is reported. The biosensor consists of a high-performance SPR imaging sensor with polarization contrast and internal referencing (refractive index resolution 2 x 10(-7) RIU) and an array of DNA probes microspotted on the surface of the SPR sensor. It is demonstrated that short sequences of nucleic acids (20-23 bases) characteristic for bacterial pathogens such as Brucella abortus, Escherichia coli, and Staphylococcus aureus can be detected at 100 pM levels. Detection of specific DNA or RNA sequences can be performed in less than 15 min by the reported SPR sensor. (C) 2008 Elsevier B.V. All rights reserved.