Whole Genome Microarray Analysis of Chicken Embryo Facial Prominences
Whole Genome Microarray Analysis of Chicken Embryo Facial Prominences
复制标题
DOI：
10.1002/dvdy.22135
复制
发表时间：
2010-02-01
影响因子：
2.5
通讯作者：
Richman, Joy M.
中科院分区：
文献类型：
--
作者：
Buchtova, Marcela;Kuo, Winston Patrick;Richman, Joy M.
面部是人类受先天缺陷影响最频繁的三个区域之一。为了了解相关的分子机制，有必要对胚胎中的基因表达有一个更完整的了解。在这里，我们使用微阵列来分析鸡面部隆起、神经脊迁移后和间充质细胞分化前的表达。芯片宽度分析显示，上颌隆起和下颌隆起具有相似的表达谱，而额鼻肿块芯片具有不同的表达谱。在面部一个或多个区域差异表达的3094个基因中，随后通过定量聚合酶链式反应(QPCR)验证了一组56个基因，并通过原位杂交检查了一个子集。微阵列趋势与大多数基因(81%)的定量聚合酶链式反应数据一致。基于定量聚合酶链式反应和微阵列数据，可以确定表征每个面部隆起的基因组。《发展动力学》239：574-591,2010。(C)2009年Wiley-Liss，Inc.
The face is one of the three regions most frequently affected by congenital defects in humans. To understand the molecular mechanisms involved, it is necessary to have a more complete picture of gene expression in the embryo. Here, we use microarrays to profile expression in chicken facial prominences, post neural crest migration and before differentiation of mesenchymal cells. Chip-wide analysis revealed that maxillary and mandibular prominences had similar expression profiles while the frontonasal mass chips were distinct. Of the 3094 genes that were differentially expressed in one or more regions of the face, a group of 56 genes was subsequently validated with quantitative polymerase chain reaction (QPCR) and a subset examined with in situ hybridization. Microarrays trends were consistent with the QPCR data for the majority of genes (81%). On the basis of QPCR and microarray data, groups of genes that characterize each of the facial prominences can be determined. Developmental Dynamics 239:574-591, 2010. (C) 2009 Wiley-Liss, Inc.