Inheritance of mitochondrial DNA and plasmids in the ascomycetous fungus, Epichloë typhina.
Inheritance of mitochondrial DNA and plasmids in the ascomycetous fungus, Epichloë typhina.
复制标题
子囊菌真菌 Epichloëtyphina 中线粒体 DNA 和质粒的遗传。
作者：
K. Chung;A. Leuchtmann;C. Schardl
分析了禾本科共生体Epichloë typhina交配过程中线粒体DNA的遗传。分析了来自杂交的80个后代，其中母本（基质）亲本具有三个线性质粒，命名为Callan-a（7.5 kb）、Aubonne-a（2.1 kb）和Bergell（2.0 kb），父本具有一个质粒Aubonne-b（2.1 kb）。在76个子代中观察到所有质粒的母体传递;两个子代具有Bergell和Callan-a，但将母体Aubonne-a替换为相关的父本质粒Aubonne-b;两个子代缺乏Callan-a，但具有另外两个母体质粒。共34个后代进行了分析，从其他四个交配，包括一个互惠对，在每个后代的质粒传输是母亲。所有后代的线粒体基因组的遗传进行了分析，限制性内切酶切割的线粒体DNA的配置文件。在大多数后代的配置文件非常相似的母本，但一些后代的非父母线粒体DNA的配置文件，建议线粒体基因组的重组。这些结果表明，E.伤寒沙门氏菌最初是异质的，允许亲本线粒体融合和它们的基因组重组。
We analyzed the inheritance of mitochondrial DNA (mtDNA) species in matings of the grass symbiont Epichloë typhina. Eighty progeny were analyzed from a cross in which the maternal (stromal) parent possessed three linear plasmids, designated Callan-a (7.5 kb), Aubonne-a (2.1 kb) and Bergell (2.0 kb), and the paternal parent had one plasmid, Aubonne-b (2.1 kb). Maternal transmission of all plasmids was observed in 76 progeny; two progeny possessed Bergell and Callan-a, but had the maternal Aubonne-a replaced with the related paternal plasmid Aubonne-b; two progeny lacked Callan-a, but had the other two maternal plasmids. A total of 34 progeny were analyzed from four other matings, including a reciprocal pair, and in each progeny the plasmid transmission was maternal. The inheritance of mitochondrial genomes in all progeny was analyzed by profiles of restriction endonuclease-cleaved mtDNA. In most progeny the profiles closely resembled those of the maternal parents, but some progeny had nonparental mtDNA profiles that suggested recombination of mitochondrial genomes. These results indicate that the fertilized stroma of E. typhina is initially heteroplasmic, permitting parental mitochondria to fuse and their genomes to recombine.