Ovarian steroids do not affect bovine endometrial cytokine or chemokine responses to Escherichia coli or LPS in vitro.
Ovarian steroids do not affect bovine endometrial cytokine or chemokine responses to Escherichia coli or LPS in vitro.
复制标题
体外卵巢类固醇不影响牛子宫内膜细胞因子或趋化因子对大肠杆菌或脂多糖的反应。
作者：
J. Saut;Gareth D Healey;Á. M. Borges;I. M. Sheldon
牛子宫内膜细菌感染导致子宫疾病的风险在卵巢周期的孕酮主导的黄体期增加，而雌激素或发情是治疗性或保护性的疾病。抵抗细菌（如引起子宫内膜炎症的大肠杆菌）的第一道防线是先天免疫系统，它识别细菌脂多糖（LPS）。本研究验证了卵巢激素浓度的周期性变化改变牛子宫内膜内先天免疫反应的假设。子宫内膜的离体器官培养物、子宫内膜上皮细胞和基质细胞以及外周血单核细胞（PBMC）的体外培养物均对E. coli或LPS诱导的IL-1β、IL-6和IL-8表达增加，IL-1B、IL-6、CXCL 8和CCL 5 mRNA表达增加。然而，这些炎症反应，典型的先天免疫，不受卵巢周期的阶段，其中子宫内膜收集器官培养，或外源性雌二醇或孕酮。虽然地塞米松阳性对照减少了E.用雌二醇或孕酮或雌二醇或孕酮核受体抑制剂处理子宫内膜细胞或PBMC，不影响IL 1 β、IL 6或IL 8的分泌，也不影响IL 1B、IL 6、CXCL 8和CCL 5基因的表达。总之，发情周期的阶段或卵巢类固醇激素并没有调节先天免疫反应在体外牛子宫内膜。
The risk of bacterial infection of the endometrium causing uterine disease in cattle is increased in the progesterone-dominated luteal phase of the ovarian cycle, while oestrogens or oestrus are therapeutic or protective against disease. The first line of defence against bacteria, such as Escherichia coli that cause inflammation of the endometrium, is the innate immune system, which recognises bacterial lipopolysaccharide (LPS). This study tested the hypothesis that cyclic variation in ovarian hormone concentrations alters innate immune responses within the bovine endometrium. Ex vivo organ cultures of endometrium, and in vitro cultures of endometrial epithelial and stromal cells, and peripheral blood mononuclear cells (PBMCs), all mounted inflammatory responses to E. coli or LPS, with secretion of inflammatory mediators interleukin 1β (IL1β), IL6 and IL8, and increased expression of mRNA encoding IL1B, IL6, CXCL8 (IL8) and CCL5. However, these inflammatory responses, typical of innate immunity, were not affected by the stage of ovarian cycle in which the endometrium was collected for organ culture, or by exogenous oestradiol or progesterone. Although a dexamethasone-positive control reduced inflammation stimulated by E. coli or LPS, treatment with oestradiol or progesterone, or inhibitors of oestradiol or progesterone nuclear receptors, did not affect endometrial cell or PBMC secretion of IL1β, IL6 or IL8, or IL1B, IL6, CXCL8 and CCL5 gene expression. In conclusion, the stage of the oestrus cycle or ovarian steroids did not modulate the innate immune response in the bovine endometrium in vitro.
DOI：
10.1530/rep-07-0229
发表时间：
2007-11
期刊：
Reproduction (Cambridge, England)
影响因子：
--
作者：
Herath S;Williams EJ;Lilly ST;Gilbert RO;Dobson H;Bryant CE;Sheldon IM
通讯作者：
Sheldon IM
影响因子：
4.8
作者：
Herath S;Lilly ST;Fischer DP;Williams EJ;Dobson H;Bryant CE;Sheldon IM
通讯作者：
Sheldon IM