Down-regulation of CEACAM1 in breast cancer
Down-regulation of CEACAM1 in breast cancer
复制标题
乳腺癌中 CEACAM1 的下调
DOI：
10.1093/abbs/gmv075
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发表时间：
2015-10-01
影响因子：
3.7
通讯作者：
Gao, Feng
中科院分区：
文献类型：
--
作者：
Yang, Changcheng;He, Pingqing;Gao, Feng
癌胚抗原相关粘附分子1（CEACAM 1）是属于CEA家族的1型跨膜糖蛋白，已发现其以可溶形式存在于体液中或以膜结合形式存在于细胞表面上。异常CEACAM 1表达与肿瘤进展相关，并且已在多种人类恶性肿瘤中发现。CEACAM 1在乳腺癌中的表达引起了越来越多的关注，但大多数研究结果是相互矛盾的。本研究的目的是更详细地研究CEACAM 1在乳腺癌中的表达。使用免疫组化染色，我们发现CEACAM 1表达减少或丢失，在乳腺癌组织相比，非癌乳腺组织。此外，乳腺癌细胞系培养基中的可溶性CEACAM 1水平显著低于非致瘤性乳腺上皮细胞系。免疫荧光分析一致地显示，乳腺癌细胞系具有相对低的膜结合CEACAM 1表达。此外，CEACAM 1 mRNA和蛋白质表达水平下调乳腺癌细胞系，分别使用实时逆转录酶-聚合酶链反应和蛋白质印迹分析。综上所述，我们的研究结果表明乳腺癌中CEACAM 1的系统性下调，并表明恢复CEACAM 1表达的策略可能有助于乳腺癌的治疗。
Carcinoembryonic antigen-related adhesion molecule 1 (CEACAM1) is a type 1 transmembrane glycoprotein belonging to the CEA family, which has been found to exist as either soluble forms in body fluids or membrane-bound forms on the cell surface. Aberrant CEACAM1 expression is associated with tumor progression and has been found in a variety of human malignancies. Increasing interest has been devoted to the expression of CEACAM1 in breast cancer, but most of these findings are contradictory. The aim of this study was to investigate CEACAM1 expression in breast cancer in greater detail. Using immunohistochemical staining, we found that CEACAM1 expression was reduced or lost in breast cancer tissues compared with noncancerous breast tissues. In addition, soluble CEACAM1 levels in the culture medium of breast cancer cell lines were significantly lower than those in a nontumorigenic breast epithelial cell line. Immunofluorescence analysis consistently showed that breast cancer cell lines have relatively low expression of membrane-bound CEACAM1. Furthermore, CEACAM1 mRNA and protein expression levels were down-regulated in breast cancer cell lines as measured using real-time reverse transcriptase-polymerase chain reaction and western blot analysis, respectively. Taken together, our results demonstrate a systematic down-regulation of CEACAM1 in breast cancer and suggest that a strategy to restore CEACAM1 expression may be helpful for the treatment of breast cancer.