Correlation of a single nucleotide polymorphism in the E-cadherin gene promoter with tumorigenesis and progression of gastric carcinoma in Japan.
Correlation of a single nucleotide polymorphism in the E-cadherin gene promoter with tumorigenesis and progression of gastric carcinoma in Japan.
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DOI：
10.3892/ijo.23.2.421
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发表时间：
2003-08
影响因子：
5.2
通讯作者：
K. Kuraoka;N. Oue;H. Yokozaki;Y. Kitadai;R. Ito;H. Nakayama;W. Yasui
中科院分区：
文献类型：
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作者：
K. Kuraoka;N. Oue;H. Yokozaki;Y. Kitadai;R. Ito;H. Nakayama;W. Yasui
E-cadherin基因启动子中的C/A单核苷酸多态性（SNP）已被证明影响体外转录效率。在这项研究中，我们分析了106例胃癌患者和90例无癌对照者的C/A SNP与临床病理参数的比较。106例胃癌患者中C/C、C/A和A/A基因型分别为61例（58%）、34例（32%）和11例（10%），90例无癌对照中分别为32例（36%）、52例（58%）和6例（6%）。胃癌患者C/C基因型明显高于无癌对照组（p<0.05）。C/C基因型的E-cadherin蛋白在非肿瘤性胃黏膜中的表达水平高于C/A基因型。在胃癌病例中，C/C基因型在低分化腺癌中（31/44;70%）明显高于高分化腺癌（30/62;48%）。C/C基因型在深部浸润（51/74;69%）和淋巴结转移（38/54;70%）患者中的表达频率显著高于浅层局限（10/32;31%）和无淋巴结转移（23/52;44%）患者（p<0.05）。这些结果提示E-cadherin启动子的C/A SNP可能是胃癌恶性程度的良好标记。
A C/A single nucleotide polymorphism (SNP) in the E-cadherin gene promoter has been shown to affect transcriptional efficiency in vitro. In this study, we analyzed the C/A SNP in 106 gastric carcinoma cases and 90 cancer-free controls compared with clinicopathological parameters. The genotype of C/C, C/A, and A/A was found in 61 (58%), 34 (32%) and 11 (10%) of 106 gastric carcinoma cases and in 32 (36%), 52 (58%) and 6 (6%) of 90 cancer-free controls, respectively. The genotype of C/C was significantly more frequent in gastric carcinoma cases than in cancer-free controls (p<0.05). The E-cadherin protein expression level in non-neoplastic gastric mucosa was higher in the C/C genotype than in the C/A. In gastric carcinoma cases, the genotype of C/C was significantly more frequent in the cases of poorly differentiated adenocarcinoma (31/44; 70%) than in well differentiated type (30/62; 48%). The C/C genotype was also significantly more frequent in the cases with deep invasion (51/74; 69%) and with lymph node metastasis (38/54; 70%) than in those confined superficially (10/32; 31%) and without lymph node metastasis (23/52; 44%), respectively (p<0.05). These results suggest that the C/A SNP in the E-cadherin promoter may be a good marker for malignancy of gastric carcinomas.