Development and use of complex probes for DNA fingerprinting the infectious fungi.
Development and use of complex probes for DNA fingerprinting the infectious fungi.
复制标题
开发和使用用于传染性真菌 DNA 指纹识别的复杂探针。
作者：
S. Lockhart;C. Pujol;S. Joly;D. Soll
已经出现了多种方法用于对感染性真菌进行遗传指纹分析。其中最通用的是Southern印迹杂交与物种特异性复杂的DNA探针，包括序列，识别高变，中度可变和不变的基因组序列。这些探针评估所有必要水平的遗传相关性，包括相同、高度相关但不相同、中度相关和不相关。描述了用于克隆复杂探针、表征它们并在不同分辨率水平验证它们的有效性的方法。已开发的用于白色念珠菌、念珠菌和念珠菌的复合探针。glabrata、光叶隐翅虫C. dubliniensis、C. tropicalis、热带假丝酵母C.近平滑和烟曲霉的描述和讨论。
A variety of methods have emerged for genetic fingerprinting the infectious fungi. One of the most versatile is Southern blot hybridization with species-specific complex DNA probes that include sequences that identify hypervariable, moderately variable and invariant genomic sequences. These probes assess genetic relatedness at all the necessary levels including identical, highly related but non-identical, moderately related and unrelated. Methods are described for cloning complex probes, characterizing them and verifying their effectiveness at the different levels of resolution. The complex probes that have been developed for Candida albicans, C. glabrata, C. dubliniensis, C. tropicalis, C. parapsilosis and Aspergillus fumigatus are described and discussed.
CARE-1 的表征：白色念珠菌重复元件-1。
影响因子：
3.5
作者：
Lasker,BA;Page,LS;Lott,TJ;Kobayashi,GS;Medoff,G
通讯作者：
Medoff,G