Identification and characterization of rat T cell subpopulations expressing T cell receptors alpha/beta and gamma/delta.
Identification and characterization of rat T cell subpopulations expressing T cell receptors alpha/beta and gamma/delta.
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表达 T 细胞受体 α/β 和 γ/δ 的大鼠 T 细胞亚群的鉴定和表征。
DOI：
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发表时间：
1990
影响因子：
5.4
通讯作者：
T. Hünig
中科院分区：
文献类型：
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作者：
A. Lawetzky;G. Tiefenthaler;R. Kubo;T. Hünig
研究了大鼠的外周淋巴器官是否存在表达泛 T 细胞标记物 CD5 和 OX-52 但不表达 T 细胞受体 (TcR) α/β 的淋巴细胞。确定了两个这样的群体：成人脾脏、淋巴结和外周血中 2% 至 4% 的淋巴细胞为 CD5+ TcR α/β-，并以与 TcR α/β+ T 细胞相同的密度表达 OX-52 抗原。这些细胞中大约 90% 是 CD8+。第二个群体是 CD5-、CD8+ 和 OX-52low。用抗 CD3 抗血清对表面标记细胞的毛地黄皂苷裂解物进行放射免疫沉淀，结果显示 TcR α/β 细胞的 CD5+ 群体表达 CD3 相关的二硫键连接的细胞表面分子，表观摩尔数约为 100 kDa，但 CD5- 群体不表达。大量的。还原后，观察到一条主要条带以 48 kDa 迁移。用抗人δ链肽抗血清获得相同大小的条带，表明CD3相关的非TcRα/β分子是大鼠TcRγ/δ。功能分析表明，大多数（如果不是全部）自然杀伤 (NK) 细胞活性存在于 CD5(-)-OX-52low 群体中。混合淋巴细胞反应中对外来主要组织相容性复合物 (MHC) 抗原的反应仅在 TcR α/β+ 脾 T 细胞中发现。结论是，大鼠脾脏中的 γ/δ T 细胞不含有大量对外来 MHC 抗原具有特异性的细胞。从出生起就在外周接种α/β和推定的γ/δ T细胞。最显着的是，在脾脏中，α/β T 细胞达到成人水平的时间比γ/δ T 细胞晚得多。总而言之，这些发现证明 TcR γ/δ 在少数具有主要表型 CD4-CD8+ 的外周大鼠 T 细胞上表达，这些细胞在新鲜分离时没有 NK 细胞活性，并且不包含高频率的同种异体反应细胞。
Peripheral lymphoid organs of the rat were investigated for the presence of lymphocytes that expressed the pan-T cell markers CD5 and OX-52 but not the T cell receptor (TcR) alpha/beta. Two such populations were identified: 2% to 4% of lymphocytes in adult spleen, lymph nodes and peripheral blood are CD5+ TcR alpha/beta- and express the OX-52 antigen at the same density as TcR alpha/beta+ T-cells. About 90% of these cells are CD8+. A second population is CD5-, CD8+ and OX-52low. Radioimmunoprecipitation from digitonin lysates of surface-labeled cells with an anti-CD3 antiserum showed that the CD5+, but not the CD5- population of TcR alpha/beta- cells expresses a CD3-associated disulfide-linked cell surface molecule of about 100 kDa apparent mol. mass. Upon reduction, one major band, migrating with 48 kDa was observed. A band of the same size was obtained with an anti-human delta chain peptide antiserum, indicating that the CD3-associated non-TcR alpha/beta molecule is the rat TcR gamma/delta. Functional assays showed that most, if not all natural killer (NK) cell activity is present in the CD5(-)-OX-52low population. Reactivity to foreign major histocompatibility complex (MHC) antigens in mixed lymphocyte reaction was exclusively found in TcR alpha/beta+ splenic T cells. It is concluded that rat gamma/delta T cells in the spleen do not contain a high frequency of cells with specificity for foreign MHC antigens. The seeding of the periphery with alpha/beta and the presumptive gamma/delta T cells was followed from birth. Most prominently in the spleen, alpha/beta T cells reached adult levels much later than gamma/delta T cells. Taken together, these findings demonstrate the expression of the TcR gamma/delta on a minor population of peripheral rat T cells with the predominant phenotype CD4-CD8+ that has no NK cell activity when freshly isolated and does not contain a high frequency of alloreactive cells.