Cleavage of double helical DNA by Cu2+ ion in the presence of bisintercalator containing penta(ethylene glycol) connector chain
Cleavage of double helical DNA by Cu2+ ion in the presence of bisintercalator containing penta(ethylene glycol) connector chain
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在含有五乙二醇连接链的双嵌入剂存在下，Cu2+ 离子对双螺旋 DNA 的裂解
DOI：
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发表时间：
1990
影响因子：
2.7
通讯作者：
M. Takagi
中科院分区：
文献类型：
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作者：
S. Takenaka;T. Ihara;M. Takagi
在设计新的DNA识别和切割试剂时，我们在此引入了双吖啶衍生物（简称双吖啶），其中两个吖啶杂环通过五（乙二醇）桥连链连接。该化合物具有两种可能的功能：1、金属离子对DNA双吖啶嵌入剂复合物的稳定作用。五（乙二醇）链稳定与DNA磷酸盐位点结合的金属离子，其中五（乙二醇）链构成用于金属结合的假大环配体的一部分;和2，通过假大环醚链的金属浓度效应增强金属辅助的DNA水解裂解。双吖啶与DNA在金属离子存在下的结合等温线表明，结合主要受阴离子DNA聚合物链上的阳离子交换反应控制，即，金属离子与阳离子双吖啶之间的交换。的双吖啶显示出增加的DNA结合能力相比，奎纳克林，单吖啶对应物，并导致在Cu 2+离子的存在下的DNA裂解的增强。其他实验包括在双吖啶存在下的DNA酶I足迹法和使用32 P末端标记的DNA片段通过Cu 2 +/双吖啶进行的DNA切割，表明在双吖啶存在下的Cu 2+辅助的DNA切割位点与双吖啶的DNA结合位点合理重叠。
In designing new DNA recognizing and cleaving reagents, we introduce herein a bisacridine derivative (referred to as bisacridine) in which two acridine heterocycles are connected by a penta(ethylene glycol) bridging chain. This compound offers two possible functions: 1, stabilization of DNA bisacridine intercalator complex by metal ion. The penta(ethylene glycol) chain stabilizes metal ions binding to the phosphate site of DNA, where the penta(ethylene glycol) chain constitutes a part of a pseudomacrocyclic ligand for metal binding; and 2, enhancement of metal‐assisted hydrolytic cleavage of DNA by means of a metal concentration effect by the pseudomacrocyclic ethereal chain. The binding isotherms of bisacridine with DNA in the presence of metal ions showed that the binding was mainly governed by the cation exchange reaction on the anionic DNA polymer chain, i.e., the exchange between metal ions and the cationic bisacridine. The bisacridine showed an increased DNA binding ability compared to quinacrine, the monoacridine counterpart, and caused an enhancement of DNA cleavage in the presence of Cu2+ ions. Additional experiments which included DNase I footprinting in the presence of bisacridine and the DNA cleavage by Cu2+/bisacridine using a 32P end‐labelled DNA fragment, suggested that the Cu2+‐assisted DNA cleavage sites in the presence of bisacridine were in reasonable overlap with the DNA binding sites of bisacridine.