Biofilms protect Mycoplasma pulmonis cells from lytic effects of complement and gramicidin
Biofilms protect Mycoplasma pulmonis cells from lytic effects of complement and gramicidin
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DOI：
10.1128/iai.00440-07
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发表时间：
2007-08-01
影响因子：
3.1
通讯作者：
Dybvig, Kevin
中科院分区：
文献类型：
--
作者：
Simmons, Warren L.;Dybvig, Kevin
肺支原体Vsa蛋白的串联重复区的长度先前已显示调节支原体对补体杀伤的敏感性：产生短形式Vsa蛋白的细胞高度敏感，而产生长Vsa蛋白的细胞具有抗性。与它们对补体的不同亲和性相反，支原体对短杆菌肽高度敏感，而与产生的Vsa蛋白的长度无关。我们在这里表明，当被包裹在生物膜中时，M。产生短形式Vsa蛋白的肺结核比分散的支原体对补体和短杆菌肽的耐药性更强。耐药性似乎局限于生物膜塔结构内的支原体。生物膜的形成可能是保护支原体免受宿主免疫的一种机制。
The length of the tandem repeat region of the Vsa protein of Mycoplasma pulmonis has previously been shown to modulate the susceptibility of mycoplasmas to killing by complement: cells that produce a short form of the Vsa protein are highly sensitive, and cells producing the long Vsa protein are resistant. In contrast to their differing susceptibilities to complement, the mycoplasmas were highly sensitive to gramicidin irrespective of the length of the Vsa protein produced. We show here that when encased within a biofilm, cells of M. pulmonis producing a short form of the Vsa protein were more resistant to complement and gramicidin than mycoplasmas that were dispersed. The resistance appeared to be localized to those mycoplasmas within tower structures of the biofilms. Biofilm formation may be a mechanism that protects mycoplasmas from host immunity.