Role of phytoplankton in maintaining endemicity and seasonality of cholera in Bangladesh
Role of phytoplankton in maintaining endemicity and seasonality of cholera in Bangladesh
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DOI：
10.1093/trstmh/trv057
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发表时间：
2015-09-01
影响因子：
2.2
通讯作者：
Collins, Andrew E.
中科院分区：
文献类型：
--
作者：
Islam, M. Sirajul;Islam, M. Shafiqul;Collins, Andrew E.
背景：在孟加拉国，霍乱是地方性的，并保持有规律的季节性模式。在孟加拉国的Matlab监测了浮游植物在维持霍乱地方性和季节性方面的作用。方法：每两周采集两个池塘的浮游植物和水样，为期1年。采用培养和直接荧光抗体技术研究了霍乱弧菌O1与浮游植物的关系。测定了水体的生物理化参数，收集了Matlab医院霍乱病例资料。采用Pearson相关系数分析了霍乱病例与浮游植物、霍乱弧菌水平及水体生物理化参数的相关性。结果：霍乱弧菌O1在可培养状态下与变异水蛭（Anabaena variabilis）联合存活48天，而在有活力但不可培养（VBNC）状态下存活1年。霍乱弧菌在对照水（无藻水）和有藻水的可培养状态下分别存活12天和32天。群落霍乱病例数的变化与水体环境中的蓝绿藻和总浮游植物有显著的相关性。霍乱病例与水生环境中叶绿素- A和VBNC霍乱弧菌O1呈显著相关。结论：本研究证明了浮游植物在维持孟加拉国霍乱的地方性和季节性方面的作用。
Background: In Bangladesh, cholera is endemic and maintains a regular seasonal pattern. The role of phytoplankton in maintaining endemicity and seasonality of cholera was monitored in Matlab, Bangladesh.Methods: Phytoplankton and water samples were collected from two ponds bi-weekly for 1 year. The association of Vibrio cholerae O1 with phytoplankton was studied by culture and direct fluorescent antibody techniques. The bio-physicochemical parameters of water were measured and data for cases of cholera were collected from the records of Matlab hospital. The correlation of cholera cases with levels of phytoplankton, V. cholerae and bio-physicochemical parameters of water was carried out using Pearson's correlation coefficients.Results: V. cholerae O1 survived for 48 days in association with Anabaena variabilis in a culturable state, but survived for a year in a viable but non-culturable (VBNC) state. V. cholerae survived for 12 and 32 days in a culturable state in control water (without algae) and water with algae, respectively. There was a significant correlation between changing levels of cholera cases in the community and the blue green algae and total phytoplankton in the aquatic environment. A significant correlation was also found between the cholera cases and chlorophyll-a and VBNC V. cholerae O1 in the aquatic environment.Conclusions: This study demonstrated the role of phytoplankton in maintaining endemicity and seasonality of cholera in Bangladesh.