ELECTRON-MICROSCOPIC ANALYSIS OF AXONAL ALTERATIONS FOLLOWING BLUNT CONTUSION OF SPINAL-CORD OF RHESUS-MONKEY (MACACA-MULATTA)
ELECTRON-MICROSCOPIC ANALYSIS OF AXONAL ALTERATIONS FOLLOWING BLUNT CONTUSION OF SPINAL-CORD OF RHESUS-MONKEY (MACACA-MULATTA)
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DOI：
10.1016/0022-510x(78)90228-9
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发表时间：
1978-01-01
影响因子：
4.4
通讯作者：
BRESNAHAN, JC
中科院分区：
文献类型：
--
作者：
BRESNAHAN, JC
本文对13只猕猴（Macaca malatta）上胸段脊髓挫伤（500 g·cm）后的脊髓切片进行了电镜观察。存活时间为4 h-10 wk。从病变部位、病变中心头侧和尾侧3 mm和10 mm区域以及腰骶髓采集样本。术后4小时，病变部位靠近灰质的几个小轴突表现出异常的细胞器积聚，包括线粒体、致密体、囊泡结构和多泡体。术后12小时，病变部位的许多轴突出现细胞器肿胀，髓鞘变薄。一些细胞器丰富的配置文件完全缺乏髓鞘。此时出现深色轴突，髓鞘似乎是空的或含有少量絮状物质。到18小时，在距病变中心3 mm的组织中出现轴突变化的第一个迹象，存在肿胀和固缩的轴突。轴突病变从脊髓的中心部分扩散到撞击部位的外周，并从撞击部位向头侧和尾侧扩散，从18 h开始并持续到研究期间。小纤维先退化，大纤维后退化。观察到的轴突变化似乎与其他种属中CNS和外周神经系统报告的变化相当。
Following contusion (500 g-cm) at upper thoracic levels, sections from the spinal cords of 13 rhesus monkeys (Macaca malatta] were examined with the EM. Survival times ranged from 4 h-10 wk. Samples were taken from the lesion site, from areas 3 and 10 mm rostral and caudal to the lesion center, and from the lumbosacral cord. Four hours postoperatively, several small axons located close to the grey matter at the lesion site exhibit abnormal accumulations of organelles including mitochondria, dense bodies, vesicular structures and multivesicular bodies. By 12 h postoperatively many axons at the lesion site appear to be swollen with organelles and exhibit thinning of their myelin sheath. Some organelle-rich profiles lack a myelin sheath altogether. At this time dark axons are present, and myelin sheaths which appear to be empty or to contain small amounts of flocculent material. By 18 h the 1st signs of axonal changes appear in the tissue taken 3 mm from the center of the lesion, both swollen and pyknotic axons being present. The axonal pathology spreads from the central part of the cord to the periphery at the impact site, and from the impact site rostrally and caudally, beginning at 18 h and continuing for the duration of the study. Small fibers degenerate first and large fibers later. The axonal changes observed appear to be comparable to those reported for the CNS and peripheral nervous systems in other species.