Detection and characterization of p44/msp2 transcript variants of Anaplasma phagocytophilum from naturally infected ticks and wild deer in Japan.
Detection and characterization of p44/msp2 transcript variants of Anaplasma phagocytophilum from naturally infected ticks and wild deer in Japan.
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DOI：
10.7883/yoken.65.79
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发表时间：
2012-01
影响因子：
2.2
通讯作者：
Gaowa;Wuritu;Dongxing Wu;Y. Yoshikawa;N. Ohashi;F. Kawamori;K. Sugiyama;M. Ohtake;M. Ohashi-M.-Ohash
中科院分区：
文献类型：
--
作者：
Gaowa;Wuritu;Dongxing Wu;Y. Yoshikawa;N. Ohashi;F. Kawamori;K. Sugiyama;M. Ohtake;M. Ohashi-M.-Ohash
嗜吞噬细胞无形体是一种专性细胞内细菌，可引起人类和牲畜的发热性疾病。在自然界中，这种细菌在蜱-哺乳动物循环中持续存在。几个p44/msp 2相关基因通过基因转换从单个表达位点表达。本研究共获得了119个来自A. 6只血蜱和3只日本野生西卡鹿（Cervus nippon）的嗜吞噬细胞菌。这119个序列根据它们的相似性分为36个不同的变异序列。36个cDNA序列在遗传学上分为2个主要簇-蜱和鹿相关。蜱相关序列进一步分为4个不同的亚群，表明A。蜱中的嗜吞噬细胞菌似乎选择性地表达特定的p44/msp 2转录物，例如与先前鉴定的p44/msp 2基因密切相关的4个亚群中的转录物。鹿相关的序列也分为4个亚群，但这些转录本可能比来自蜱的转录本更多样化。这可能是由于p44/msp 2在鹿中的相对非选择性表达或A.来自不同地理区域的蜱和鹿或两者的嗜吞噬细胞菌。因此，本研究有助于对A. p44/msp 2在日本自然界中的表达。
Anaplasma phagocytophilum is an obligate intracellular bacterium and causes a febrile illness in humans and livestock. In nature, this bacterium is sustained in a tick-mammal cycle. Several p44/msp2-related genes are expressed from a single expression locus by gene conversion. In this study, we obtained 119 cDNA sequences of p44/msp2 transcripts from A. phagocytophilum in 6 Haemaphysalis ticks and 3 wild sika deer (Cervus nippon) in Japan. These 119 sequences were classified into 36 different variant sequences based on their similarities. The 36 cDNA sequences were phylogenetically grouped into 2 major clusters--tick- and deer-associated. The tick-associated sequences were further classified into 4 distinct subclusters, suggesting that A. phagocytophilum in ticks seems to selectively express specific p44/msp2 transcripts, such as the transcripts in the 4 subclusters that were closely related to previously identified p44/msp2 genes. The deer-associated sequences were also grouped into 4 subclusters, but these transcripts were probably more diverse than the transcripts derived from ticks. This might be due to the relatively nonselective expression of p44/msp2 in deer or the strain differences in A. phagocytophilum from ticks and deer in separate geographic regions or both. Thus, this study may contribute to the understanding of A. phagocytophilum p44/msp2 expression in nature in Japan.