Human arthroplasty derived macrophages differentiate into osteoclastic bone resorbing cells
Human arthroplasty derived macrophages differentiate into osteoclastic bone resorbing cells
复制标题
DOI：
10.1136/ard.56.7.414
复制
发表时间：
1997-07-01
影响因子：
27.4
通讯作者：
Athanasou, NA
中科院分区：
文献类型：
--
作者：
Sabokbar, A;Fujikawa, Y;Athanasou, NA
在无菌性松动中，关节置换术组织中通常发现对生物材料磨损颗粒的严重巨噬细胞反应。本研究的目的是发现这些细胞是否通过分化成破骨细胞而促进无菌性松动的骨吸收。方法在翻修手术时，从松质骨水泥和非骨水泥髋关节置换术的假囊和假膜中分离巨噬细胞，然后在玻璃盖玻片和牙本质切片上与UMR 106大鼠成骨细胞样细胞共培养，25二羟维生素D-3 [1，25（OH）（2）D-3]。巨噬细胞分离的滑膜骨关节炎（OA）患者接受髋关节置换术进行了类似的研究作为对照group.Results-After 24小时孵育，大多数细胞分离的上述假体周围组织强烈表达巨噬细胞（CD 11b，CD 14），但不是破骨细胞标记。然而，孵育14天后，在存在1，25（OH）（2）D-3的情况下，在关节成形术衍生的巨噬细胞和UMR 106细胞的共培养物中形成了大量显示破骨细胞表型特征的多核细胞（即，抗酒石酸酸性磷酸酶、玻连蛋白受体阳性，并能够进行广泛的腔隙性吸收）。添加巨噬细胞集落刺激因子（M-CSF）抗体可显著降低巨噬细胞-破骨细胞分化，因此在这些共培养物中观察到腔隙性吸收。与此相反，OA滑膜巨噬细胞/UMR 106共培养物显示很少或没有证据表明巨噬细胞破骨细胞分化，这是只有当人类M-CSF被添加到co-culture. Conclusion，这是第一次报告显示，人类巨噬细胞分离直接从周围松动的植入物假体周围组织分化成多核细胞，显示所有的功能和细胞化学特性的破骨细胞。与其他巨噬细胞群体相比，这种情况的发生不需要外源性M-CSF。在假体周围组织中，巨噬细胞对磨损颗粒有强烈反应，巨噬细胞-破骨细胞分化可能代表了无菌性松动中骨质溶解的重要细胞机制。
Objective-In aseptic loosening, a heavy macrophage response to biomaterial wear particles is commonly found in arthroplasty tissues. The aim of this study was to discover if these cells contribute to the bone resorption of aseptic loosening by differentiating into osteoclasts.Methods-Macrophages were isolated from the pseudocapsule and pseudomembrane of loose cemented and uncemented hip arthroplasties at the time of revision surgery and then co-cultured on glass coverslips and dentine slices with UMR 106 rat osteoblast-like cells, both in the presence and absence of 1,25 dihydroxyvitamin D-3 [1,25(OH)(2)D-3]. Macrophages isolated from the synovial membrane of patients with osteoarthritis (OA) undergoing hip replacements were similarly studied as a control group.Results-After 24 hours incubation, most cells isolated from the above periprosthetic tissues strongly expressed macrophage (CD11b, CD14) but not osteoclast markers. However, after 14 days incubation, numerous multinucleated cells showing the phenotypic features of osteoclasts (that is, positive for tartrate resistant acid phosphatase, the vitronectin receptor, and capable of extensive lacunar resorption) formed in co-cultures of arthroplasty derived macrophages and UMR 106 cells, in the presence of 1,25(OH)(2)D-3. The addition of an antibody to macrophage colony stimulating factor (M-CSF) considerably reduced macrophage-osteoclast differentiation and hence the lacunar resorption seen in these co-cultures. In contrast, OA synovial macrophage/UMR 106 co-cultures showed little or no evidence of macrophage-osteoclast differentiation and this was only seen when human M-CSF was added to the co-cultures.Conclusion-This is the first report showing that human macrophages isolated directly from periprosthetic tissues surrounding loosened implants differentiate into multinucleated showing all the functional and cytochemical characteristics of osteoclasts. In contrast with other macrophage populations, exogenous M-CSF is not required for this to occur. In the context of the heavy macrophage response to wear particles in periprosthetic tissues macrophage-osteoclast differentiation may represent an important cellular mechanism whereby osteolysis is effected in aseptic loosening.