Effects of caloric restriction on mitochondrial function and gene transcripts in rat muscle.
Effects of caloric restriction on mitochondrial function and gene transcripts in rat muscle.
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热量限制对大鼠肌肉线粒体功能和基因转录的影响。
DOI：
10.1152/ajpendo.00387.2001
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Nair,KSreekumaran
中科院分区：
文献类型：
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作者：
Sreekumar,R;Unnikrishnan,J;Fu,A;Nygren,J;Short,KR;Schimke,J;Barazzoni,R;Nair,KSreekumaran
啮齿动物骨骼肌线粒体 DNA 已被证明是衰老过程中潜在的氧化损伤部位。据报道，热量限制（CR）可以减少啮齿动物的氧化应激并延长预期寿命。在给雄性大鼠喂食对照饮食或热量限制饮食（对照饮食的 60%）36 周后，对它们的骨骼肌进行基因表达谱分析和线粒体 ATP 生产能力测量，以确定 CR 有益作用的潜在机制。 CR增强了参与活性氧自由基清除功能、组织发育和能量代谢的基因的转录，同时降低了参与信号转导、应激反应以及结构和收缩蛋白的基因的表达。实时 PCR 测量证实了微阵列方法注意到的细胞色素氧化酶 III、超氧化物歧化酶 (SOD)1 和 SOD2 转录水平的变化。线粒体 ATP 生成和柠檬酸合酶未因饮食变化而改变。我们得出的结论是，CR 改变了骨骼肌中几个基因的转录水平，并且骨骼肌中的线粒体功能仍未因饮食干预而改变。许多参与活性氧清除功能的基因转录本的改变可能有助于 CR 寿命的延长。
Rodent skeletal muscle mitochondrial DNA has been shown to be a potential site of oxidative damage during aging. Caloric restriction (CR) is reported to reduce oxidative stress and prolong life expectancy in rodents. Gene expression profiling and measurement of mitochondrial ATP production capacity were performed in skeletal muscle of male rats after feeding them either a control diet or calorie-restricted diet (60% of control diet) for 36 wk to determine the potential mechanism of the beneficial effects of CR. CR enhanced the transcripts of genes involved in reactive oxygen free radical scavenging function, tissue development, and energy metabolism while decreasing expression of those genes involved in signal transduction, stress response, and structural and contractile proteins. Real-time PCR measurments confirmed the changes in transcript levels of cytochrome-coxidase III, superoxide dismutase (SOD)1, and SOD2 that were noted by the microarray approach. Mitochondrial ATP production and citrate synthase were unaltered by the dietary changes. We conclude that CR alters transcript levels of several genes in skeletal muscle and that mitochondrial function in skeletal muscle remains unaltered by the dietary intervention. Alterations in transcripts of many genes involved in reactive oxygen scavenging function may contribute to the increase in longevity reported with CR.