Free cysteine is increased in plasma from hemodialysis patients
Free cysteine is increased in plasma from hemodialysis patients
复制标题
DOI：
10.1046/j.1523-1755.2003.00808.x
复制
发表时间：
2003-03-01
影响因子：
19.6
通讯作者：
Takamitsu, Y
中科院分区：
文献类型：
--
作者：
Nakanishi, T;Hasuike, Y;Takamitsu, Y
背景。虽然在以前的大多数临床研究中，只有总硫醇浓度（包括结合形式和自由形式）被确定，但自由形式可能是心血管风险的更好预测指标。我们测量了健康对照者和慢性肾功能衰竭患者血液透析前后血浆中滤过部分和酸溶部分中游离同型半胱氨酸（Hcy）和半胱氨酸（Cys）的表观浓度。在对照组中，滤过的Hcy和酸溶性Hcy相似，但滤过的Cys比酸溶性Cys小得多。在透析前样品中，过滤后的Cys丰度（259.2 +/- 26.2 mumol/L）是对照样品（4.1 +/- 0.7 mumol/L）的60倍以上。对照组滤过Cys的游离总比值为1.6 +/- 0.3%，而透析前患者为40.9 +/- 2.7%。硫醇的过滤分数可以用来估计溶质通过透析膜的运输。此外，半胱氨酸可能参与血液透析患者动脉粥样硬化的发病机制，应重新研究。
Background. Although only the total thiol concentration, which includes bound and free forms, has been determined in most previous clinical studies, the free form may be a better predictor of cardiovascular risk.Methods. We measured the apparent concentration of free homocysteine (Hcy) and cysteine (Cys) in filtered and acid-soluble fractions of plasma in healthy control subjects and in patients with chronic renal failure just before and after a hemodialysis session.Results. In control, filtered Hcy and acid-soluble Hcy were similar, while filtered Cys was much smaller than in acid-soluble Cys. In prehemodialysis samples, filtered Cys was more than 60 times as abundant (259.2 +/- 26.2 mumol/L) as in control samples (4.1 +/- 0.7 mumol/L ). Free-to-total ratios for filtered Cys were 1.6 +/- 0.3% in controls, but 40.9 +/- 2.7% in prehemodialysis patients.Conclusions. The filtered fraction of thiols can be used to estimate solute transport across the dialysis membrane. In addition, the possible involvement of cysteine in the pathogenesis of atherosclerosis in hemodialysis patients should be reexamined.