Imaging Transcription: Past, Present, and Future.
Imaging Transcription: Past, Present, and Future.
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DOI：
10.1101/sqb.2015.80.027201
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Lionnet T
中科院分区：
文献类型：
--
作者：
Coleman RA;Liu Z;Darzacq X;Tjian R;Singer RH;Lionnet T
转录是基因表达的第一步，在高等真核生物中受到精细的调控，以确保正确的发育和稳态。传统的生物化学、遗传学和基因组学方法已被证明在鉴定调节转录的因子、调控序列和潜在途径方面是成功的。然而，它们通常仅提供涉及转录调控的高度动态、随机生化过程的快照或群体平均值。因此，单分子活细胞成像已经成为能够规避这些限制的补充方法。通过观察分子事件在其天然环境中发生时的真实的时间序列，成像具有导出因果关系和定量动力学以建立转录的预测模型的能力。荧光成像技术的不断进步带来了新的显微镜和标记技术，现在可以在活细胞和动物中以单分子分辨率可视化和量化转录过程。在这里，我们提供了转录成像技术的发展和现状的概述。我们讨论了他们发现的一些重要概念，并提出了未来可能的发展，可能会解决长期存在的问题，转录调控。
Transcription, the first step of gene expression, is exquisitely regulated in higher eukaryotes to ensure correct development and homeostasis. Traditional biochemical, genetic, and genomic approaches have proved successful at identifying factors, regulatory sequences, and potential pathways that modulate transcription. However, they typically only provide snapshots or population averages of the highly dynamic, stochastic biochemical processes involved in transcriptional regulation. Single molecule live-cell imaging has, therefore, emerged as a complementary approach capable of circumventing these limitations. By observing sequences of molecular events in real time as they occur in their native context, imaging has the power to derive cause-and-effect relationships and quantitative kinetics to build predictive models of transcription. Ongoing progress in fluorescence imaging technology has brought new microscopes and labeling technologies that now make it possible to visualize and quantify the transcription process with single-molecule resolution in living cells and animals. Here we provide an overview of the evolution and current state of transcription imaging technologies. We discuss some of the important concepts they uncovered and present possible future developments that might solve long-standing questions in transcriptional regulation.