The interferon receptors.
The interferon receptors.
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DOI：
10.1002/0471684228.egp06422
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
M. Rubinstein;P. Orchansky
中科院分区：
文献类型：
--
作者：
M. Rubinstein;P. Orchansky
对干扰素作用方式的早期研究表明，细胞表面的受体系统参与其作用。第一个直接的证据，以高亲和力结合位点被发现后，纯干扰素是可用的。识别出两种不同的受体，一种对干扰素-α和β具有特异性，另一种对干扰素-γ具有特异性。建立了与受体的亲和力和比活性之间的相关性。标记干扰素与其受体的交联复合物在凝胶电泳上可见，甚至部分纯化。报道了干扰素与受体结合后的内化。最近研究了神经节苷脂作为干扰素结合辅助物的作用。干扰素片段仍然保留了结合能力的描述，并有助于阐明干扰素分子上的结合位点。
Early studies on the mode of action of interferons have indicated that a receptor system on the cell surface is involved in its action. The first direct evidence to a high-affinity binding site was found only after pure interferon was available. Two different receptors, one specific for interferons-alpha and beta, and the other for interferon-gamma were recognized. A correlation between affinity to the receptor and specific activity was established. Cross-linked complexes of labeled interferons with their receptors were visualized on gel electrophoresis and even partially purified. Internalization of interferons after binding to the receptor was reported. The role of gangliosides as helpers of interferon binding was recently investigated. Fragments of interferons which still retained binding capacity were described and helped in elucidating the binding site on the interferon molecule.