Isolation and characterization of a cDNA encoding a Translin-like protein, TRAX
Isolation and characterization of a cDNA encoding a Translin-like protein, TRAX
复制标题
DOI：
10.1016/s0014-5793(96)01444-5
复制
发表时间：
1997-01-20
期刊：
影响因子：
3.5
通讯作者：
Kasai, M
中科院分区：
文献类型：
--
作者：
Aoki, K;Ishida, R;Kasai, M
Translin是一种DNA结合蛋白，在许多淋巴细胞恶性肿瘤中特异性结合染色体易位断点连接处的一致序列。为了研究其在这些重组热点中的功能意义，我们使用酵母双杂交系统检测了Translin是否与其他蛋白相互作用，并鉴定了一个相关的33 kd蛋白伙伴TRAX，它具有广泛的氨基酸同源性。TRAX蛋白在其n端区域含有两部分核靶向序列，提示缺乏任何核靶向基序的Translin蛋白可能在选择性核转运中起作用。
Translin is a DNA binding protein which specifically binds to consensus sequences at breakpoint junctions of chromosomal translocations in many cases of lymphoid malignancies, To investigate its functional significance at such recombination hotspots, we examined whether Translin interacts with other proteins using a yeast two-hybrid system and identified an associated 33 kd protein partner, TRAX, with extensive amino acid homology, The TRAX protein was established to contain bipartite nuclear targeting sequences in its N-terminal region, suggesting a possible role in the selective nuclear transport of Translin protein lacking any nuclear targeting motifs.