The novel ABC transporter ABCH1 is a potential target for RNAi-based insect pest control and resistance management.
The novel ABC transporter ABCH1 is a potential target for RNAi-based insect pest control and resistance management.
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DOI：
10.1038/srep13728
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发表时间：
2015-09-03
影响因子：
4.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Guo Z;Kang S;Zhu X;Xia J;Wu Q;Wang S;Xie W;Zhang Y
害虫对作物造成严重危害，并对化学杀虫剂和苏云金芽孢杆菌(Bt)杀虫哭泣毒素产生高度抗性。RNA干扰(RNAi)是控制和克服这种抗性的一个新的有前途的方法。基于RNAi的昆虫控制策略依赖于选择合适的靶基因。在本研究中，我们克隆并鉴定了小菜蛾ABC转运蛋白基因PxABCH1。系统发育分析表明，PxABCH1与ABCA和ABCG亚家族成员关系密切。时空表达检测表明，PxABCH1在各组织和发育阶段均有表达，以头部和雄性成体表达最高。所有敏感和抗性小菜蛾品系的中肠序列变异和PxABCH1基因的表达分析以及亚致死量RNAi功能分析表明，Cry1Ac抗性与该基因无关。用相对较高剂量的dsRNA沉默PxABCH1，显著降低了PxABCH1的表达，并导致敏感和抗Cry1Ac小菜蛾品系的幼虫和幼虫死亡表型。据我们所知，这项研究首次深入了解了鳞翅目昆虫中的ABCH1，并揭示了它是基于RNAi的害虫控制和抗性管理的极佳靶标。
Insect pests cause serious crop damage and develop high-level resistance to chemical insecticides and Bacillus thuringiensis (Bt) insecticidal Cry toxins. A new promising approach for controlling them and overcoming this resistance is RNA interference (RNAi). The RNAi-based insect control strategy depends on the selection of suitable target genes. In this study, we cloned and characterized a novel ABC transporter gene PxABCH1 in diamondback moth, Plutella xylostella (L.). Phylogenetic analysis showed that PxABCH1 is closely related to ABCA and ABCG subfamily members. Spatial-temporal expression detection revealed that PxABCH1 was expressed in all tissues and developmental stages, and highest expressed in head and male adult. Midgut sequence variation and expression analyses of PxABCH1 in all the susceptible and Bt-resistant P. xylostella strains and the functional analysis by sublethal RNAi demonstrated that Cry1Ac resistance was independent of this gene. Silencing of PxABCH1 by a relatively high dose of dsRNA dramatically reduced its expression and resulted in larval and pupal lethal phenotypes in both susceptible and Cry1Ac-resistant P. xylostella strains. To our knowledge, this study provides the first insight into ABCH1 in lepidopterans and reveals it as an excellent target for RNAi-based insect pest control and resistance management.