siRNA Suppression of NEDD9 Inhibits Proliferation and Enhances Apoptosis in Renal Cell Carcinoma.
siRNA Suppression of NEDD9 Inhibits Proliferation and Enhances Apoptosis in Renal Cell Carcinoma.
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DOI：
10.3727/096504015x14386062091442
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发表时间：
2014
影响因子：
3.1
通讯作者：
Kong F
中科院分区：
文献类型：
--
作者：
Wang J;Yang WJ;Sun C;Luan Y;Cheng GH;Li KL;Kong F
肾细胞癌是所有泌尿生殖系统恶性肿瘤中最致命的。NEDD9/HEF1/Cas- l是Cas蛋白家族的成员，被认为是多种癌症类型的生物标志物。在这项研究中，我们首次证明了NEDD9在RCC组织和细胞系中上调。免疫组织化学分析和定量RT-PCR分析显示，NEDD9在正常肾组织中低表达，在RCC组织中高表达。此外，体外实验表明，NEDD9在RCC细胞系中表达上调。通过MTT实验，我们观察到NEDD9敲低抑制了细胞的增殖。此外，流式细胞术分析显示NEDD9下调可诱导细胞凋亡。总之，我们的数据表明，异常的NEDD9蛋白表达可能是RCC的一个标志，而NEDD9敲低会抑制细胞生长。
Renal cell carcinoma (RCC) is the most lethal of all genitourinary malignancies. NEDD9/HEF1/Cas-L is a member of the Cas protein family and is known as a biomarker in multiple cancer types. In this study, we demonstrate for the first time that NEDD9 was upregulated in RCC tissue and cell lines. Immunohistochemical analysis and quantitative RT-PCR analysis showed low expression of NEDD9 in normal renal tissues and high expression in RCC tissues. In addition, in vitro experiments show that expression of NEDD9 was upregulated in RCC cell lines. Through MTT assay, we observed that NEDD9 knockdown inhibited cell proliferation. Furthermore, flow cytometry analysis showed that NEDD9 downregulation induced apoptosis. Together, our data suggest that abnormal NEDD9 protein expression may be a marker for RCC, and NEDD9 knockdown suppresses cell growth.