The earliest stages of B cell development require a chemokine stromal cell-derived factor/pre-B cell growth-stimulating factor
The earliest stages of B cell development require a chemokine stromal cell-derived factor/pre-B cell growth-stimulating factor
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DOI：
10.1016/s1074-7613(01)00185-6
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发表时间：
2001-08-01
期刊：
影响因子：
32.4
通讯作者：
Nagasawa, T
中科院分区：
文献类型：
--
作者：
Egawa, T;Kawabata, K;Nagasawa, T
B淋巴细胞生成的第一阶段所必需的环境因素仍然难以捉摸。在这里，我们报告说，立即承诺后，B谱系，前体成为依赖于趋化因子SDF-1及其受体CXCR 4使用突变和辐射嵌合小鼠。在骨髓中，最早可识别的B细胞前体群体的产生需要CXCR 4。在胎肝中，我们鉴定了Lin（-）CD 19（-）c-kit（+）IL 7 R alpha（+）AA 4.1（+），这是最早的单能B细胞前体群体，并发现其发育在SDF-1（-/-）胚胎中受到严重影响，但在IL-7（-/-）胚胎中不受影响。在SDF-1（-/-）胚胎中Lin（-）T细胞祖细胞出现正常。此外，SDF-1对最早期的B细胞前体表现出特异性生物活性。SDF-1提供了负责最早B谱系阶段的细胞因子的第一个实例。
Environmental factors essential for the first stages of B lymphopoiesis remain elusive. Here, we report that immediately after commitment to B lineage, precursors become dependent on a chemokine SDF-1 and its receptor CXCR4 using mutant and radiation chimeric mice. In bone marrow, generation of the earliest identifiable B cell precursor populations requires CXCR4. In fetal liver, we identified Lin(-)CD19(-)c-kit(+)IL7R alpha (+)AA4.1(+), the earliest unipotent B cell precursor population, and found that its development was severely affected in SDF-1(-/-) embryos but not in IL-7(-/-) embryos. Lin(-) T cell progenitors appeared normal in SDF-1(-/-) embryos. Moreover, SDF-1 exhibited specific biologic activities on the earliest B cell precursors. SDF-1 provides the first example of a cytokine responsible for the earliest B lineage stages.