CHARACTERIZATION AND EXPRESSION OF RPOC2 IN CMS AND FERTILE LINES OF SORGHUM
CHARACTERIZATION AND EXPRESSION OF RPOC2 IN CMS AND FERTILE LINES OF SORGHUM
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DOI：
10.1007/bf00042066
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发表时间：
1995-08-01
影响因子：
5.1
通讯作者：
HART, GE
中科院分区：
文献类型：
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作者：
CHEN, ZJ;SCHERTZ, KF;HART, GE
在 CMS 高粱（Sorghum bicolor (L.)）的小花药类型中，鉴定出 rpoC2（编码 RNA 聚合酶 β'' 亚基的质体基因）中部的 165 bp 缺失。 Moench，含有A1、A2、A5和A6细胞质。之前的研究表明，这些 CMS 系中删除的氨基酸序列位于单子叶植物特异性区域，该区域包含几个蛋白质基序，这些蛋白质基序是多个转录因子的特征。使用 PCR 分析中缺失侧翼的引物，将各种类型的 CMS 系（其中一些用于杂交高粱生产）分为两组。含有A1、A2、A5、A6细胞质的CMS系显示rpoC2中的缺失。这些品系具有小的花药，其中花粉发育在早期阶段被抑制并且通常仅发现空的外壁。含有 A3、A4 和 9E 细胞质的 CMS 系不具有缺失。这些品系具有较大的花药，其中花粉在后期退化。使用 15 个叶绿体基因进行的连续转录测定表明，CMS 和可育（维持系和恢复系）系以及幼苗叶片中的 F-1 中的叶绿体基因转录率相似。 RNA印迹分析表明，rbcL、rpoB和rpoC2转录本主要在叶片中积累，在花序组织和花粉中含量较低。这些数据记录了来自 CMS 和高粱可育细粉的叶子和非光合组织中质体基因的表达。
A 165 bp deletion in the middle of rpoC2, the plastid gene which encodes the RNA polymerase beta '' subunit, was identified in the small-anthered types of CMS sorghum, Sorghum bicolor (L.). Moench, containing Al, A2, A5, and A6 cytoplasms. It was previously shown that the amino acid sequence deleted in these CMS lines is in a monocot-specific region that contains several protein motifs that are characteristic of several transcription factors. Using primers flanking the deletion in PCR analyses, various types of CMS lines, some of which are used in hybrid sorghum production, were classified into two groups. CMS lines containing Al, A2, A5, A6 cytoplasms display the deletion in rpoC2. These lines have small anthers in which pollen development is arrested at an early stage and in which usually only empty exines are found. CMS lines containing A3, A4, and 9E cytoplasms do not possess the deletion. These lines have large anthers in which pollen degenerates at a later stage. Run-on transcription assays using 15 chloroplast genes showed that chloroplast gene transcription rates are similar in CMS and fertile (maintainer and restorer) lines and F-1 in seedling leaves. Analyses of RNA blots indicated that rbcL, rpoB and rpoC2 transcripts are accumulated mainly in the leaves and low in the inflorescence tissues and pollen. These data document plastid gene expression in leaves and non-photosynthetic tissues from CMS and fertile fines of sorghum.