Genetic and physical mapping of the grapevine powdery mildew resistance gene, Run1, using a bacterial artificial chromosome library
Genetic and physical mapping of the grapevine powdery mildew resistance gene, Run1, using a bacterial artificial chromosome library
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DOI：
10.1007/s00122-005-2030-8
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发表时间：
2005-07-01
影响因子：
5.4
通讯作者：
Dry, I
中科院分区：
文献类型：
--
作者：
Barker, CL;Donald, T;Dry, I
葡萄藤白粉病的抗性由Run1控制，Run1是存在于野生葡萄藤种圆叶葡萄（Muscadinia rotundifolia）中的单一显性基因，但不存在于栽培种葡萄属葡萄（Vinifera）中。已使用假回交策略将运行1基因渗入酿酒葡萄中，并且先前已鉴定出与抗性基因座连锁的遗传标记。在这里，我们描述了建设全面的遗传和物理地图跨越抗性基因座，使未来的定位克隆的抗性基因。使用细菌人工染色体（BAC）文库进行物理作图，所述细菌人工染色体（BAC）文库使用从在基因渗入内携带Runl的抗性酿酒葡萄个体提取的基因组DNA构建。BAC重叠群组装已经使20个新的遗传标记被鉴定为与Run1紧密连锁，并且抗性位点的位置已经被细化，将基因定位在简单序列重复（SSR）标记VMC4f3.1和BAC末端序列衍生标记CB 292.294之间。该区域包含两个多基因家族的抗性基因类似物（RGA）。物理和遗传作图数据的比较表明，重组在Run1附近受到严重抑制，这可能是由于来自M.带有Run1基因的圆叶植物。
Resistance to grapevine powdery mildew is controlled by Run1, a single dominant gene present in the wild grapevine species, Muscadinia rotundifolia, but absent from the cultivated species, Vitis vinifera. Run1 has been introgressed into V. vinifera using a pseudo-backcross strategy, and genetic markers have previously been identified that are linked to the resistance locus. Here we describe the construction of comprehensive genetic and physical maps spanning the resistance locus that will enable future positional cloning of the resistance gene. Physical mapping was performed using a bacterial artificial chromosome (BAC) library constructed using genomic DNA extracted from a resistant V. vinifera individual carrying Run1 within an introgression. BAC contig assembly has enabled 20 new genetic markers to be identified that are closely linked to Run1, and the position of the resistance locus has been refined, locating the gene between the simple sequence repeat (SSR) marker, VMC4f3.1, and the BAC end sequence-derived marker, CB292.294. This region contains two multigene families of resistance gene analogues (RGA). A comparison of physical and genetic mapping data indicates that recombination is severely repressed in the vicinity of Run1, possibly due to divergent sequence contained within the introgressed fragment from M. rotundifolia that carries the Run1 gene.