Comparison among fecal secondary bile acid levels, fecal microbiota and Clostridium scindens cell numbers in Japanese
Comparison among fecal secondary bile acid levels, fecal microbiota and Clostridium scindens cell numbers in Japanese
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DOI：
10.1111/j.1348-0421.2004.tb03526.x
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发表时间：
2004-01-01
影响因子：
2.6
通讯作者：
Benno, Y
中科院分区：
文献类型：
--
作者：
Kitahara, M;Sakata, S;Benno, Y
通过肠道细菌的胆汁酸7 α-脱羟基化，其将胆酸和鹅去氧胆酸分别转化为脱氧胆酸（DCA）和石胆酸（LCA），是人肠道中的重要功能。Clostridium glandens是胆汁酸7 α-脱羟基化的重要菌种之一。黄茅具有高水平的胆汁酸7 α-脱羟基化活性。我们量化了C。结果表明，40例健康日本人粪便中的胆汁酸、二次胆汁酸、DCA和LCA存在显著性差异，并探讨了它们的相关性。此外，我们使用末端限制性片段长度多态性（T-RFLP）分析来研究粪便微生物群对次级胆汁酸水平的影响。C.粪便样本中的胆汁酸和次级胆汁酸。另一方面，T-RFLP分析表明，与高水平DCA相关的粪便微生物群与低水平DCA相关的粪便微生物群不同，此外，老年人（72岁以上）的粪便微生物群与年轻人（55岁以下）的粪便微生物群显著不同。这些结果表明，肠道微生物群对DCA水平的影响比C的数量更强。使细胞肿胀。
Bile acid 7alpha-dehydroxylation by intestinal bacteria, which converts cholic acid and chenodeoxycholic acid to deoxycholic acid (DCA) and lithocholic acid (LCA), respectively, is an important function in the human intestine. Clostridium scindens is one of the most important bacterial species for bile acid 7alpha-dehydroxylation because C. scindens has high levels of bile acid 7alpha-dehydroxylating activity. We quantified C. scindens and secondary bile acids, DCA and LCA, in fecal samples from 40 healthy Japanese and investigated their correlation. Moreover, we used terminal restriction fragment length polymorphism (T-RFLP) analysis to investigate the effect of fecal microbiota on secondary bile acid levels. There was no correlation between C. scindens and secondary bile acid in fecal samples. On the other hand, T-RFLP analysis demonstrated that fecal microbiota associated with high levels of DCA were different from those associated with low levels of DCA, and furthermore that fecal microbiota in the elderly (over 72 years) were significantly different from those in younger adults (under 55 years). These results suggest that intestinal microbiota have a stronger effect on DCA level than does the number of C. scindens cells.