Fluorescence anisotropy: analysis of tRNA binding to the T box riboswitch antiterminator RNA.

Fluorescence anisotropy: analysis of tRNA binding to the T box riboswitch antiterminator RNA.
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荧光各向异性:分析 tRNA 与 T 盒核糖开关抗终止子 RNA 的结合。

DOI:
10.1007/978-1-4939-1896-6_11
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发表时间:
2015
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Hines,JV
Hines,JV
中科院分区:
--
文献类型:
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作者:
Zhou,S;Anupam,R;Hines,JV

文献摘要

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Fluorescence anisotropy can be utilized in drug discovery screening assays to identify compounds that disrupt medicinally important RNA–macromolecular complexes. Here we describe the application of this technique to monitor tRNA binding to T box riboswitch antiterminator RNA.