Regeneration of active endogenous IAA in rice calli following acclimation to 2,4-D free medium
Regeneration of active endogenous IAA in rice calli following acclimation to 2,4-D free medium
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水稻愈伤组织在 2,4-D 无培养基驯化后活性内源 IAA 的再生
DOI：
10.1007/s10725-020-00679-0
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发表时间：
2020-11-07
影响因子：
4.2
通讯作者：
Cai, Weiming
中科院分区：
文献类型：
--
作者：
Jin, Jing;Essemine, Jemaa;Cai, Weiming
在无生长素的培养基上生长的适应性水稻愈伤组织是从先前在补充有2，4-D的培养基上培养的水稻胚来源的愈伤组织中分离的。驯化的水稻愈伤组织比未驯化的愈伤组织生长快，特别是在第一周。我们进行了微阵列分析，以解开在无淀粉培养基上生长的水稻愈伤组织的转录水平的变化。几个与激素代谢和信号转导相关的基因在两种愈伤组织类型之间差异表达。特别是，与未驯化的愈伤组织相比，驯化的愈伤组织中两个酰胺转移酶基因Gretchen哈根3s（GH3.1和GH3.8）的表达下调，一个吲哚-3-乙酸（IAA）-氨基酸（IAA-AA）水解酶基因ILR 1-like 3的表达上调。我们进一步评估了这两种愈伤组织中不同激素的含量。IAA含量确实显着增加了约7倍，在习惯性愈伤组织相比，非习惯性，最初生长在植物无糖培养基。与内源生长素、IAA-AA结合物、脱落酸（阿坝）、乙烯和GA（12）、GA（19）、GA（29）含量的增加相平行，驯化后的愈伤组织中内源激素含量减少。我们的研究结果表明，内源生长素的再生是必不可少的，所需的愈伤组织细胞的习惯。在适应过程中，内源激素的变化可能通过多种生物学途径进行协调和监测。最后，对愈伤组织适应过程中内源激素的变化和调节进行了讨论。本报告还显示出完全不同的激素组成的状态为每个水稻愈伤组织，这可能是潜在的负责愈伤组织的生长调节和驯化过程。
The habituated rice calli grown on an auxin-free medium were isolated from rice embryo-derived calli previously cultivated on medium supplemented with 2,4-D. The habituated rice calli grow faster than the non-habituated ones, particularly during the first week. We performed microarray analysis to unravel the changes in the transcriptomic level of rice calli grown on hormone-free medium. Several genes related to hormone metabolism and signaling were differentially expressed between the two calli types. Especially, the expressions of two amido-transferase enzymes genes Gretchen Hagen 3s (GH3.1 and GH3.8) were down-regulated and one indole-3-acetic acid (IAA)-amino acid (IAA-AA) hydrolase gene ILR1-like 3 was up-regulated in habituated calli compared to non-habituated. We further assessed the contents of different hormones in these two calli kinds. The IAA content was indeed significantly increased by about 7 times in habituated calli compared to non-habituated, initially grown in plant hormone-free medium. In parallel to the increase in the endogenous auxin, IAA-AA conjugates, abscisic acid (ABA), ethylene and GA(12), GA(19), GA(29) were decreased in the habituated calli. Our results indicate that the regeneration of endogenous auxin is essential and required for the habituation of callus cells. The changes in the endogenous hormones might be coordinated and monitored during the habituation process by various biological pathways. Eventually, this study debates the interrelated changes and adjustment in endogenous plant hormones occurred (happened) in the habituated calli. This report exhibits also a completely different hormonal composition status for each rice calli, which might potentially be responsible for the calli growth regulation and its acclimation process.