ORIGIN OF ALVEOLAR MACROPHAGES IN MOUSE RADIATION CHIMERAS
ORIGIN OF ALVEOLAR MACROPHAGES IN MOUSE RADIATION CHIMERAS
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DOI：
10.1084/jem.136.3.630
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发表时间：
1972-01-01
影响因子：
15.3
通讯作者：
BRAIN, JD
中科院分区：
文献类型：
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作者：
GODLESKI, JJ;BRAIN, JD
本研究试图确定肺泡巨噬细胞的主要来源是肺还是造血。我们利用抗原标记物来鉴别造血细胞的来源。小鼠嵌合体是通过照射C57B6/ af1小鼠（900 R），然后静脉注射B10D2/ af1骨髓产生的。供体动物在h -2位点上具有抗原特异性，而受体不具有这种特异性。37℃下用生理盐水反复洗肺获得肺泡巨噬细胞。采用小鼠抗31抗体、兔吸收血清作为补体、台盼蓝排斥法对骨髓和肺泡巨噬细胞进行细胞毒试验。分别在照射和骨髓置换后7、14、21、28和35-50天以及4、5、8和11个月对动物进行研究。照射后21天，90%的小鼠骨髓置换率超过80%；白细胞和肺泡巨噬细胞计数接近正常。在这个时候和以后的时间间隔内，肺游离细胞群中供体细胞的百分比与骨髓中供体细胞的百分比没有显著差异。同样，暴露于气溶胶颗粒后，供体来源的骨髓细胞和肺泡巨噬细胞的百分比无显著差异。这种免疫学方法表明，辐射嵌合体中的肺泡巨噬细胞完全是造血起源的。
This investigation attempted to determine whether the primary source of alveolar macrophages is pulmonary or hematopoietic. We have utilized an antigenic marker to identify cells of hematopoietic origin. Mouse chimeras were produced by irradiating C57B6/AF1mice (900 R) and then injecting them intravenously with B10D2/AF1bone marrow. The donor animal has an antigenic specificity on theH-2locus, not shared by the recipient. Alveolar macrophages were obtained by repeated lung washings with physiologic saline at 37°C. Cytotoxic tests were done on bone marrow and alveolar macrophages using anti-31 mouse antibody, absorbed rabbit serum as complement, and trypan blue exclusion as a test for viability. Animals were studied at 7, 14, 21, 28, and 35–50 days and 4, 5, 8, and 11 months after irradiation and bone marrow replacement. By 21 days after irradiation, 90% of the animals had greater than 80% replacement of marrow with donor tissue; and white blood cell and alveolar macrophage counts approached normal. At this time and at later intervals the per cent of donor cells in the lung free cell population was not significantly different from the per cent of donor cells in the bone marrow. Similarly, after aerosol particulate exposure, the percentage of marrow cells and alveolar macrophages of donor origin were not significantly different. This immunologic approach suggests that alveolar macrophages in radiation chimeras are entirely of hematopoietic origin.