Ligand binding analysis of soluble interleukin-2 receptor complexes by surface plasmon resonance.
Ligand binding analysis of soluble interleukin-2 receptor complexes by surface plasmon resonance.
复制标题
通过表面等离子体共振对可溶性白细胞介素 2 受体复合物进行配体结合分析。
DOI：
10.1074/jbc.270.27.16045
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ciardelli,TL
中科院分区：
文献类型：
--
作者：
Wu,Z;Johnson,KW;Choi,Y;Ciardelli,TL
Knowledge of the kinetic binding characteristics is often critical to the development of ligand/receptor structure-activity relationships. To better understand the contribution of each of the subunits to ligand binding in the multimeric interleukin-2 receptor system, we have previously prepared stable solution complexes of the α- and β-subunits. In this study, we have employed surface plasmon resonance biosensor methodology (BIAcore™) to evaluate both the kinetic and equilibrium binding constants for these complexes. The structural nature of the complexes facilitated immobilization on the sensor surfaces in a manner that minimized interference with ligand interactions. The interleukin-2 receptor complex surfaces displayed excellent binding capacity and stability toward regeneration. In all cases where the binding constants were measurable, the values determined for interleukin-2 were in good agreement with those previously determined by other methods. When interleukin-2 analogs with receptor subunit specific mutations were employed, the binding parameters were consistent with the nature of the mutations. The combination of coiled-coil-mediated solution assembly and surface plasmon resonance analysis of ligand binding provides a powerful approach to the study of multimeric cytokine receptor systems.
