A marine bacterial community capable of degrading poly(ethylene terephthalate) and polyethylene
A marine bacterial community capable of degrading poly(ethylene terephthalate) and polyethylene
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DOI：
10.1016/j.jhazmat.2021.125928
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发表时间：
2021-04-30
影响因子：
13.6
通讯作者：
Sun, Chaomin
中科院分区：
文献类型：
--
作者：
Gao, Rongrong;Sun, Chaomin
塑料废物正在成为最常见的海洋废弃物形式，并构成日益严重的全球污染问题。在这里，我们对数百个与塑料废物相关的样品进行了筛选，发现了一种能够有效地定殖和降解聚对苯二甲酸乙二醇酯（PET）和聚乙烯（PE）的海洋细菌群落。使用绝对定量16 S rRNA测序和培养方法，我们获得了相应的丰度和纯化培养物的三个细菌菌株介导的塑料降解。我们进一步进行了许多技术，以表征PET和PE的有效降解的重组细菌群落含有这三种细菌。此外，我们使用液相色谱-质谱法来进一步证明PET和PE膜的降解由重建的细菌群落。我们进行了转录组学的方法来研究塑料降解过程和潜在的降解机制介导的我们重建的细菌群落。最后，我们基于转录组学结果过表达PE降解酶，并验证了它们对PE膜的显著降解作用。总的来说，我们的研究建立了一个稳定的海洋细菌群落，可以有效地降解PET和PE，并为塑料降解途径及其相关的生物和机械过程提供了见解，为开发针对塑料废物的微生物产品铺平了道路。
Plastic wastes are becoming the most common form of marine debris and present a growing global pollution problem. Here, we used a screening approach on hundreds of plastic waste-associated samples and discovered a marine bacterial community capable of efficiently colonizing and degrading both poly(ethylene terephthalate) (PET) and polyethylene (PE). Using absolute quantitative 16S rRNA sequencing and cultivation methods, we obtained corresponding abundance and purified cultures of three bacterial strains that mediated plastic degradation. We further performed numerous techniques to characterize the efficient degradation of PET and PE by the reconstituted bacterial community containing these three bacteria. Additionally, we used liquid chromatography-mass spectrometry to further demonstrate the degradation of PET and PE films by the reconstituted bacterial community. We conducted transcriptomic methods to investigate the plastic degradation process and potential degradation mechanisms mediated by our reconstituted bacterial community. Lastly, we overexpressed PE degradation enzymes based on transcriptomic results and verified their significant degradation effects on the PE films. Overall, our study establishes a stable marine bacterial community that efficiently degrades PET and PE and provides insights into plastic degradation pathways and their associated biological and mechanistic processes-paving the way for developing microbial products against plastic wastes.