Surface and virulence properties of environmental Vibrio cholerae non-O1 from Albufera Lake (Valencia, Spain)
Surface and virulence properties of environmental Vibrio cholerae non-O1 from Albufera Lake (Valencia, Spain)
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阿尔布费拉湖（西班牙巴伦西亚）环境霍乱弧菌非 O1 的表面和毒力特性
DOI：
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发表时间：
1990
影响因子：
4.4
通讯作者：
E. Garay
中科院分区：
文献类型：
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作者：
C. Amaro;A. E. Toranzo;E. A. González;J. Blanco;M. Pujalte;R. Aznar;E. Garay
共140个环境霍乱弧菌非O1分离株，连同几个文化收集菌株从环境和临床来源，进行了研究，有关血凝，表面疏水性，和酶，溶血，细胞毒性和肠毒性活性的细胞外产物。共78和62%的菌株产生血凝素和外血凝素，分别。四个不同的血凝和两个exohemagglutinating活动被发现通过使用八种糖的抑制试验。细胞结合甘露糖敏感的血凝反应主要在鸡血液中检测到，而岩藻糖敏感的血凝反应只记录在人血液中。细胞结合血凝素耐所有糖测试是唯一一个相关的表面疏水性。表面性质沿着生长曲线变化。非O1菌株表现出较强的酶和溶血活性，除了七叶苷水解。在26个选择用于细胞毒素和肠毒素生产的非O1分离株中，23个对变温和恒温种属的细胞系表现出广谱的细胞毒性作用，但它们在幼鼠试验中具有弱肠毒素原性。所有细胞毒菌株的胞外产物都是蛋白水解的，脂肪分解的，溶血的，并且高百分比产生的鸡血液凝集。所分析的非O1菌株中的细胞毒因子不是R质粒介导的。
A total of 140 environmental Vibrio cholerae non-O1 isolates, together with several culture collection strains from both environmental and clinical sources, were studied in relation to hemagglutination, surface hydrophobicity, and the enzymatic, hemolytic, cytotoxic, and enterotoxic activities of their extracellular products. A total of 78 and 62% of the strains produced hemagglutinins and exohemagglutinins, respectively. Four different hemagglutinating and two exohemagglutinating activities were found by using eight sugars in the inhibition assays. Cell-bound mannose-sensitive hemagglutination was detected mainly in chicken blood, whereas fucose-sensitive hemagglutination was recorded only in human blood. Cell-bound hemagglutinin resistant to all sugars tested was the only one related to surface hydrophobicity. The surface properties varied along the growth curves. The non-O1 strains displayed strong enzymatic and hemolytic activities, except for esculin hydrolysis. Of 26 non-O1 isolates selected for cytotoxin and enterotoxin production, 23 showed a wide spectrum of cytotoxic effects on cell lines of poikilothermic and homoiothermic species, but they were weakly enterotoxigenic in the infant mouse test. All extracellular products of cytotoxic strains were proteolytic, lipolytic, and hemolytic, and a high percentage produced hemagglutination of chicken blood. The cytotoxic factors in the non-O1 strains analyzed were not R plasmid mediated.