Role of NAD(+)-dependent 'malic' enzyme and pyruvate dehydrogenase complex in leaf metabolism
Role of NAD(+)-dependent 'malic' enzyme and pyruvate dehydrogenase complex in leaf metabolism
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DOI：
10.1042/bst0240743
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发表时间：
1996-08-01
影响因子：
3.9
通讯作者：
Leaver, C
中科院分区：
文献类型：
--
作者：
Hill, S;Winning, B;Leaver, C
“这项工作的目的是研究三羧酸（TCA）循环在光照光合组织中的作用。人们通常认为光照成熟叶片的 ATP 需求是通过光磷酸化来满足的，这意味着线粒体不活跃。最近已经清楚地证明线粒体 ATP 合成在叶代谢中起着至关重要的作用。应用所选择的寡霉素浓度，仅抑制线粒体而不是质体 ATP 合成，已被证明可以通过降低 ATP 合成速率来降低光合作用速率 [13，支持早期的结果，即光呼吸过程中甘氨酸氧化释放的还原能力可以与 ATP 偶联
‘I’he aim of this work is to investigate the role of the tricarboxylic acid (TCA) cycle in illuminated photosynthetic tissues. It is often assumed that the ATP requirements of the illuminated mature leaf are met by photophosphorylation, implying that mitochondria are inactive. Recently it has been clearly demonstrated that mitochondrial ATP synthesis plays a crucial role in leaf metabolism. The application of concentrations of oligomycin chosen so that only mitochondrial and not plastidic ATP synthesis is inhibited has been shown to reduce the rate of photosynthesis, via a reduction in the rate of ATP synthesis [13, supporting earlier results that suggested that the reducing power released by glycine oxidation during photorespiration could be coupled to ATP