Differentiation of myeloid dendritic cells into CD8α-positive dendritic cells in vivo
Differentiation of myeloid dendritic cells into CD8α-positive dendritic cells in vivo
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DOI：
10.1182/blood.v96.5.1865.h8001865_1865_1872
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发表时间：
2000-09-01
期刊：
影响因子：
20.3
通讯作者：
Engleman, EG
中科院分区：
文献类型：
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作者：
Merad, M;Fong, L;Engleman, EG
骨髓来源的树突状细胞（DC）代表了具有不同表型的抗原呈递细胞（APC）家族。例如，在小鼠中，CD8 α(+)和CD8 α (-) DC被认为分别代表淋巴细胞和髓细胞起源。表皮的朗格汉斯细胞（LC）为典型的髓系DC；它们不表达CD8 α，但表达高水平的髓系抗原，如CD11b和Fc γ R。相比之下，源自淋巴相关祖细胞的胸腺DC表达CD8 α，但仅表达低水平的髓系抗原，CD8 α (+) DC也存在于脾脏和淋巴结（LN）中，但这些细胞的来源尚未确定。通过激活和标签CD8α(-)表皮LC体内,发现这些细胞表达CD8α迁移到排水LN,同样,CD8α(-)LC CD8野生型小鼠的体外生成和注入皮肤CD8αKO小鼠表达CD8α当他们到达排水LN,结果还表明,αCD8(+)信用证是强有力的APC,从皮肤移植后,他们在t细胞LN、局部分泌interleukin-12水平高,干扰素和趋化因子吸引T细胞，它们诱导抗原特异性T细胞活化。这些结果表明，外周的髓性DC在迁移到引流LN时可以表达CD8 α， CD8 α在这些DC上的表达似乎反映了激活、动员或两者的状态，而不是谱系。(C) 2000年由美国血液学会出版。
Bone marrow-derived dendritic cells (DC) represent a family of antigen-presenting cells (APC) with varying phenotypes. For example, in mice, CD8 alpha(+) and CD8 alpha(-) DC are thought to represent cells of lymphoid and myeloid origin, respectively. Langerhans cells (LC) of the epidermis are typical myeloid DC; they do not express CD8 alpha, but they do express high levels of myeloid antigens such as CD11b and Fc gamma R, By contrast, thymic DC, which derive from a lymphoid-related progenitor, express CD8 alpha but only low levels of myeloid antigens, CD8 alpha(+) DC are also found in the spleen and lymph nodes (LN), but the origin of these cells has not been determined. By activating and labeling CD8 alpha(-) epidermal LC in vivo, it was found that these cells expressed CD8 alpha on migration to the draining LN, Similarly, CD8 alpha(-) LC generated in vitro from a CD8 wild-type mouse and injected into the skin of a CD8 alpha KO mouse expressed CD8 alpha when they reached the draining LN, The results also show that CD8 alpha(+) LC are potent APC, After migration from skin, they localized in the T-cell areas of LN, secreted high levels of interleukin-12, interferon-gamma, and chemokine-attracting T cells, and they induced antigen-specific T-cell activation. These results demonstrate that myeloid DC in the periphery can express CD8 alpha when they migrate to the draining LN, CD8 alpha expression on these DC appears to reflect a state of activation, mobilization, or both, rather than lineage. (C) 2000 by The American Society of Hematology.