Extra- and intracellular water spaces in muscles of man at rest and with dynamic exercise.
Extra- and intracellular water spaces in muscles of man at rest and with dynamic exercise.
复制标题
人在休息和动态运动时肌肉中的细胞外和细胞内水空间。
DOI：
--
复制
发表时间：
1982
影响因子：
--
通讯作者：
B. Saltin
中科院分区：
文献类型：
--
作者：
G. Sjøgaard;B. Saltin
建立了一种分析小肌肉组织样品中细胞外水空间（H2Oe）作为[3 H]菊粉分布空间的方法。在对大鼠进行初步实验后，该方法应用于13名男性和6名女性。在休息时研究了具有不同纤维组成的肌肉（比目鱼肌，S;股外侧肌，（VL;腓肠肌，G;肱三头肌，TB）。所有肌肉的总含水量相同，为320（313-330）ml/100 g干重。然而，H2Oe表现出差异，VL中为26-34 ml/100 g干重，S中为38-54 ml/100 g干重（P小于0.05）; G和TB的值介于VL和S之间。H2Oe的差异与肌肉的纤维组成无关。在3 x 3 min的高强度自行车运动中，VL的总含水量从313 ml/100 g干重增加到359 ml/100 g干重，H2Oe从34 ml/100 g干重增加到60 ml/100 g干重（P <0.05）。计算的细胞内乳酸盐浓度在VL中从5.7 mmol/l H2 O2增加至30.6 mmol/l H2 O2。运动结束时细胞外乳酸浓度为13.6 mmol/l H2Oe。乳酸2从内到外的空间的浓度梯度有利于水通量的细胞内空间。因此，H2Oe的相对较大的增加可能是由流体静力学因素而不是渗透因素引起的。
A method was established to analyze the extracellular water space (H2Oe) in small muscle tissue samples as [3H]inulin distribution space. After initial experiments on rats, the method was applied on 13 men and 6 women. Muscles with different fiber compositions (soleus, S; vastus lateralis, (VL; gastrocnemius, G; triceps brachii, TB) were studied at rest. The total water content was the same for all muscles, 320 (313-330) ml/100 g dry wt. However, differences were demonstrated for H2Oe, with 26-34 ml/100 g dry wt in VL and 38-54 ml/100 g dry wt in S, (P less than 0.05); the values for G and TB were in between those for VL and S. The differences in H2Oe were not related to the fiber composition of the muscles. During 3 x 3 min of intense bicycle exercise demanding about 120% VO2 max (6 men), total water content increased in VL from 313 to 359 ml/100 g dry wt and H2Oe increased from 34 to 60 ml/100 g dry wt (P less than 0.05), In TB, which is relatively inactive during bicycle exercise, no such changes occurred. The calculated intracellular lactate concentration increased in VL from 5.7 to 30.6 mmol/l H2Oi. The extracellular lactate concentration amounted to 13.6 mmol/l H2Oe at the end of exercise. The concentration gradient for lactate of 2 from intra- to extracellular space favored a flux of water to the intracellular space. The relative large increase in H2Oe may then be caused by a hydrostatic rather than an osmotic factor.U