Endurance training increases stimulation of uncoupling of skeletal muscle mitochondria in humans by non-esterified fatty acids: an uncoupling-protein-mediated effect?
Endurance training increases stimulation of uncoupling of skeletal muscle mitochondria in humans by non-esterified fatty acids: an uncoupling-protein-mediated effect?
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耐力训练增加非酯化脂肪酸对人体骨骼肌线粒体解偶联的刺激：解偶联蛋白介导的效应？
DOI：
10.1042/bj3510805
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Kent Sahlin
中科院分区：
文献类型：
--
作者：
M. Tonkonogi;Anna Krook;Brandon Walsh;Kent Sahlin
非耦合呼吸(UCR)是肌肉线粒体的一种基本特性，在细胞内具有多种功能。我们假设耐力训练可能会改变人类肌肉中UCR的大小和性质。在6周耐力训练前和6周后(n=8)对肌肉活检组织中分离的线粒体进行UCR分析。为了探讨解偶联蛋白2(UCP2)和解偶联蛋白3(UCP3)在解偶联蛋白2(UCP2)和解偶联蛋白3(UCP3)中的作用，检测了UCP调节配体(非酯化脂肪酸和嘌呤核苷酸)和UCP2和UCP3在肌肉中表达的敏感性。油酸增加线粒体耗氧速率，这种作用不被GDP和/或环孢素A所减弱，与训练前相比，油酸的作用显著增强。运动对UCP2和UCP3基因表达水平无明显影响，但运动后油酸诱导的呼吸频率相对增加与UCP2基因表达水平呈正相关。综上所述，我们发现耐力训练上调了UCR对非酯化脂肪酸的敏感性。这表明耐力训练引起线粒体功能的内在变化，这可能增强调节有氧能量产生的潜力，防止过量自由基的产生，并有助于更高的基础代谢率。
Uncoupled respiration (UCR) is an essential property of muscle mitochondria and has several functions in the cell. We hypothesized that endurance training may alter the magnitude and properties of UCR in human muscle. Isolated mitochondria from muscle biopsies taken before and after 6 weeks of endurance exercise training (n=8) were analysed for UCR. To investigate the role of uncoupling protein 2 (UCP2) and UCP3 in UCR, the sensitivity of UCR to UCP-regulating ligands (non-esterified fatty acids and purine nucleotides) and UCP2 and UCP3 mRNA expression in muscle were examined. Oleate increased the mitochondrial oxygen consumption rate, an effect that was not attenuated by GDP and/or cyclosporin A. The effect of oleate was significantly greater after compared with before training. Training had no effect on UCP2 or UCP3 mRNA levels, but after training the relative increase in respiration rate induced by oleate was positively correlated with the UCP2 mRNA level. In conclusion, we show that the sensitivity of UCR to non-esterified fatty acids is up-regulated by endurance training. This suggests that endurance training causes intrinsic changes in mitochondrial function, which may enhance the potential for regulation of aerobic energy production, prevent excess free radical generation and contribute to a higher basal metabolic rate.
脂肪酸激活重建的棕色脂肪组织线粒体解偶联蛋白。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Strieleman,PJ;Schalinske,KL;Shrago,E
通讯作者：
Shrago,E