Wiring Specificity and Synaptic Diversity in the Mouse Lateral Central Amygdala
Wiring Specificity and Synaptic Diversity in the Mouse Lateral Central Amygdala
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DOI：
10.1523/jneurosci.3309-15.2016
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发表时间：
2016-04-20
影响因子：
5.3
通讯作者：
Lien, Cheng-Chang
中科院分区：
文献类型：
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作者：
Hou, Wen-Hsien;Kuo, Ning;Lien, Cheng-Chang
中央杏仁核（CeA）是一种皮质下结构，主要由GABA释放（GABA能）神经元组成，通过投射到下丘脑和脑干中的下游靶点来控制恐惧表达。CeA由外侧（CeL）和内侧（CeM）细分组成。CeL强烈门信息传输到CeM，杏仁核的主要输出站，但很少有人知道在这个地区的本地电路的功能组织。使用聚类分析，我们确定了两个主要的电生理不同的细胞神经元类在小鼠杏仁核切片，早期尖峰（ES）和晚期尖峰（LS）神经元。这两个类显示出不同的autaptic传输。与LS神经元相比，ES神经元具有强烈的抑制性自适应，提高了发放时间的精确性。通过多次膜片钳记录，我们发现CeL神经元产生化学突触，但不产生电突触。个体连接的分析揭示了大麻素1型受体介导的ES抑制，但不是LS细胞输出突触。更有趣的是，ES->LS或LS->ES突触的功效类似于LS-->LS或ES-->ES突触的功效的2倍。当在20 Hz下测试时，不同神经元之间的突触，但不是在同一类别内，明显令人沮丧，并且更强大地塑造突触后神经元的活动。此外，不同类别的神经元也形成具有更高连接度的突触。我们表明，ES和LS神经元代表两个功能不同的细胞类的细胞和突触前和突触后神经元之间的相互作用决定神经元之间的突触特性。
The central amygdala (CeA) nucleus, a subcortical structure composed of mostly GABA-releasing (GABAergic) neurons, controls fear expression via projections to downstream targets in the hypothalamus and brainstem. The CeA consists of the lateral (CeL) and medial (CeM) subdivisions. The CeL strongly gates information transfer to the CeM, the main output station of the amygdala, but little is known about the functional organization of local circuits in this region. Using cluster analysis, we identified two major electrophysiologically distinct CeL neuron classes in mouse amygdala slices, the early-spiking (ES) and late-spiking (LS) neurons. These two classes displayed distinct autaptic transmission. Compared with LS neurons, ES neurons had strong and depressing autapses, which enhanced spike-timing precision. With multiple patch-clamp recordings, we found that CeL neurons made chemical, but not electrical, synapses. Analysis of individual connections revealed cannabinoid type 1 receptor-mediated suppression of the ES, but not of the LS cell output synapse. More interestingly, the efficacy of the ES->LS or LS->ES synapse was similar to 2-fold greater than that of the LS-->LS or ES-->ES synapse. When tested at 20 Hz, synapses between different neurons, but not within the same class, were markedly depressing and were more powerful to sculpt activity of postsynaptic neurons. Moreover, neurons of different classes also form synapses with higher degree of connectivity. We demonstrate that ES and LS neurons represent two functionally distinct cell classes in the CeL and interactions between presynaptic and postsynaptic neurons dictate synaptic properties between neurons.