Immunohistochemical Identification of Canine Melanocytic Neoplasms With Antibodies to Melanocytic Antigen PNL2 and Tyrosinase: Comparison With Melan A
Immunohistochemical Identification of Canine Melanocytic Neoplasms With Antibodies to Melanocytic Antigen PNL2 and Tyrosinase: Comparison With Melan A
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DOI：
10.1177/0300985810382095
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发表时间：
2011-03-01
影响因子：
2.4
通讯作者：
Miller, M. A.
中科院分区：
文献类型：
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作者：
Ramos-Vara, J. A.;Miller, M. A.
将PNL 2和抗酪氨酸酶在福尔马林固定、石蜡包埋的犬黑素细胞肿瘤（n = 101）中的免疫反应性与Melan A进行比较。在总共113个样品中，106个对PNL 2呈阳性，101个对Melan A呈阳性，90个对酪氨酸酶呈阳性。6例PNL 2阳性的黑素瘤对Melan A呈阴性; 1例PNL 2阴性的黑素瘤对Melan A呈阳性。80例肿瘤对所有3种标记物均呈阳性; 111例与3种抗体中的至少1种反应。用甲酸脱钙长达1周并不影响任何标记物的免疫反应性;然而，用盐酸脱钙1天或1周显着降低或完全消除了Melan A和PNL 2的免疫反应性。在用盐酸脱钙1周的组织中，酪氨酸酶的免疫反应性只有轻微的损失。长期固定（长达2个月）不影响PNL 2或酪氨酸酶免疫反应性;然而，固定1个月后，Melan A免疫反应性降低。PNL 2在120个非黑素细胞肿瘤（癌、肉瘤、类固醇产生肿瘤和白细胞肿瘤）中不表达。总之，在犬黑素细胞肿瘤的鉴定中，抗体PNL 2比Melan A稍微更敏感，并且比酪氨酸酶更敏感。此外，PNL 2似乎与非黑素细胞肿瘤没有交叉反应。PNL 2耐长时间固定，但对强脱钙敏感。结果表明，PNL 2是一个很好的标记物，在犬黑色素瘤的鉴定和灵敏度接近100%，当与Melan A和酪氨酸酶结合使用。
The immunoreactivity of PNL2 and antityrosinase in formalin-fixed, paraffin-embedded canine melanocytic neoplasms (n = 101) was compared with that of Melan A. Of the 113 samples overall, 106 were positive for PNL2, 101 for Melan A, and 90 for tyrosinase. Six melanomas that were positive for PNL2 were negative for Melan A; 1 melanoma that was negative for PNL2 was positive for Melan A. Eighty tumors were positive for all 3 markers; 111 reacted with at least 1 the 3 antibodies. Decalcification with formic acid for up to 1 week did not affect immunoreactivity of any of the markers; however, decalcification with HCl for 1 day or 1 week notably decreased or completely abrogated immunoreactivity for Melan A and PNL2. There was only minor loss of immunoreactivity for tyrosinase in tissues decalcified with HCl for 1 week. Prolonged fixation (up to 2 months) did not affect PNL2 or tyrosinase immunoreactivity; however, Melan A immunoreactivity was reduced after 1 month of fixation. PNL2 was not expressed in 120 nonmelanocytic tumors (carcinomas, sarcomas, steroid-producing tumors, and leukocytic tumors). In summary, antibody PNL2 is slightly more sensitive than Melan A and more sensitive than tyrosinase in the identification of canine melanocytic neoplasms. Furthermore, PNL2 does not appear to cross-react with nonmelanocytic neoplasms. PNL2 is resistant to prolonged fixation but sensitive to strong decalcification. Results indicate that PNL2 is an excellent marker in the identification of canine melanomas and that the sensitivity is close to 100% when used in conjunction with Melan A and tyrosinase.