Evaluation of flagellum-related proteins FliD and FspA as subunit vaccines against Campylobacter jejuni colonisation in chickens.
Evaluation of flagellum-related proteins FliD and FspA as subunit vaccines against Campylobacter jejuni colonisation in chickens.
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DOI：
10.1016/j.vaccine.2016.02.052
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发表时间：
2016-04-04
期刊：
影响因子：
5.5
通讯作者：
Stevens MP
中科院分区：
文献类型：
--
作者：
Chintoan-Uta C;Cassady-Cain RL;Stevens MP
弯曲杆菌是发达国家人类食源性腹泻的主要原因，食用受污染的禽肉是感染的主要来源。肉鸡接种疫苗可减少肉鸡胴体污染和人畜共患感染。为此，我们评估了基于鞭毛覆盖蛋白FliD和鞭毛分泌蛋白FspA的重组抗弯曲杆菌亚单位疫苗，因为它们在鸡体内具有免疫原性，而鞭毛对定植至关重要。在三项研究中，重组FliD疫苗在孵卵当天初次接种后49天，诱导盲肠定植水平短暂但可重复且具有统计学意义的降低c. 2 log10 CFU/g。在个体鸟类中，flidd特异性血清IgY水平与盲肠细菌计数呈正相关，表明此类抗体可能不发挥保护作用。这些数据增加了用于控制一种关键食源性人畜共患病的有限候选抗原库。
Campylobacter is the leading cause of food-borne diarrhoea in humans in the developed world and consumption of contaminated poultry meat is the main source of infection. Vaccination of broilers could reduce carcass contamination and zoonotic infections. Towards this aim, we evaluated recombinant anti-Campylobacter subunit vaccines based on the flagellum-capping protein FliD and the flagellum-secreted protein FspA as they are immunogenic in chickens and the flagellum is vital for colonisation. In three studies, a recombinant FliD vaccine induced a transient but reproducible and statistically significant decrease of c. 2 log10 CFU/g in caecal colonisation levels at 49 days post-primary vaccination on the day of hatch. Levels of serum IgY specific to FliD positively correlated with caecal bacterial counts in individual birds, indicating that such antibodies may not play a role in protection. The data add to the limited repertoire of candidate antigens for the control of a key foodborne zoonosis.