Expression patterns of neurotrophic factor mRNAs in developing human teeth
Expression patterns of neurotrophic factor mRNAs in developing human teeth
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DOI：
10.1007/s00441-002-0618-8
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发表时间：
2002-11-01
影响因子：
3.6
通讯作者：
Nosrat, CA
中科院分区：
文献类型：
--
作者：
Nosrat, I;Seiger, Å;Nosrat, CA
神经营养因子调节神经系统的存活、分化、生长和可塑性。此外，基于其特定的和变化的时空表达模式，神经营养因子已被牵连在啮齿动物牙齿发育过程中的形态发生事件。为了确定啮齿类动物中的这些发现是否与人类有关，我们现在研究了神经生长因子（NGF）、脑源性神经营养因子（BDNF）、神经营养素-3（NT-3）、神经营养素-4（NT-4）、胶质细胞系源性神经营养因子（GDNF）和neurturin（NTN）mRNA的表达。妊娠6.5-11周人类牙齿发育模式。使用原位杂交组织化学，我们发现不同的和特定的模式，神经营养素和GDNF mRNA的表达在发育中的人牙齿。NGFmRNA标记较弱，主要局限于牙乳头。脑源性神经营养因子mRNA标记比神经生长因子mRNA强，并且可见于牙器官侧面的间充质以及上皮结构（内牙上皮和釉结）中。NT-3 mRNA在牙乳头和颈袢区均有表达。NT-4 mRNA在口腔和牙齿上皮中的表达在研究的各个阶段。GDNF mRNA在牙囊和内牙上皮的不同部位均有表达。在发育中的牙齿中也发现了弱的NTN mRNA标记。基于这些发现，我们认为，神经营养因子，GDNF和NTN可能参与在人类牙齿发育的早期阶段的形态发生事件。蛋白基因产物（PGP）9.5-免疫反应性神经纤维在牙囊中观察到的11周，与神经营养因子mRNA的标记在这个结构相一致。提示这些神经营养因子可能参与牙体结构的神经支配。神经营养因子在发育中的牙组织中的丰富表达表明，发育中的牙组织或可能的成人牙组织可用作神经系统疾病的营养支持的同种异体移植物来源。
Neurotrophic factors regulate survival, differentiation, growth and plasticity in the nervous system. In addition, based on their specific and shifting temporospatial expression patterns, neurotrophic factors have been implicated in morphogenetic events during tooth development in rodents. To determine whether these findings in rodents could be related to humans, we have now studied nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), glial cell-line derived neurotrophic factor (GDNF), and neurturin (NTN) mRNA expression. patterns in developing human teeth during gestational weeks 6.5-11. Using in situ hybridization histochemistry, we found distinct and specific patterns of neurotrophin and GDNF mRNA expression in the developing human teeth. NGF mRNA labeling was weak and confined predominantly to the dental papilla. BDNF mRNA labeling was stronger than NGF mRNA and was seen in the mesenchyme located lateral to the dental organ, as well as in epithelial structures (inner dental epithelium and enamel knot). NT-3 mRNA was observed in the dental papilla and in the area of the cervical loop. NT-4 mRNA was expressed in both oral and dental epithelia in all stages studied. GDNF mRNA was found in the dental follicle and at different sites in the inner dental epithelium. Weak NTN mRNA labeling was also found in the developing teeth. Based on these findings, we suggest that neurotrophins, GDNF and NTN might be involved in morphogenetic events during early stages of tooth development in humans. Protein gene product (PGP) 9.5-immunoreactive nerve fibers were observed in the dental follicle by 11 weeks coinciding with the labeling for neurotrophic factor mRNAs in this structure. This suggests that these neurotrophic factors might be involved in the innervation of dental structures. The rich expression of neurotrophic factors in developing dental tissues suggests that developing, or possibly adult, dental tissue might be used as an allograft source of trophic support for diseases of the nervous system.